LOCUS       sequence-167404-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-670-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    151..285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(188..207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(595..616)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaattcaaaa ttttatcgat cacgagacta gcctcgagag atctgatcat aatcagccat
       61 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      121 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      181 aaataaggca atagcatcac aaatttcaca aataaggcat ttttttcact gcattctagt
      241 tttggtttgt ccaaactcat caatgtatct tatcatgtct ggatctcaaa tccctcggaa
      301 gctgcgcctg tcatcgaatt cctgcagccc ggtgcatgac taagctagta ccggtgtccc
      361 tgagtgtatg tggtggtccc tgagtgtatg tggtggtccc tgagtgtatg tggtggtccc
      421 tgagtgtatg tggtggtccc tgagtgtatg tggtggtccc tgagtgtatg tggtggctac
      481 gacgattggt actccccagt taaggtcggc tagctcagtt agcctccccc atctctcgac
      541 gcggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      601 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
      661 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      721 gtagtggtc
//
