LOCUS       sequence-167407-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-668-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    137..271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(174..193)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(575..596)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcgatcacg agactagcct cgagagatct gatcataatc agccatacca catttgtaga
       61 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      121 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaggcaatag
      181 catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg gtttgtccaa
      241 actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg cgcctgtcat
      301 cgaattcctg cagcccggtg catgactaag ctagtaccgg ttgcgcctcg ggtgagcatg
      361 tgcgcctcgg gtgagcatgt gcgcctcggg tgagcatgtg cgcctcgggt gagcatgtgc
      421 gcctcgggtg agcatgtgcg cctcgggtga gcatggctac gacgattggt actccccagt
      481 taaggtcggc tagctcagtt agcctccccc atctctcgac gcggccgctt tacttgtaca
      541 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
      601 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg
      661 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
      721 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
      781 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
      841 tgttgccgtc ctccttgaag t
//
