LOCUS       sequence-167415-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-920.sensor.PGK.dNGFR....
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    138..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(175..194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(582..603)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatcgatcac gagactagcc tcgagagatc tgatcataat cagccatacc acatttgtag
       61 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      121 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaggcaata
      181 gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt ggtttgtcca
      241 aactcatcaa tgtatcttat catgtctgga tctcaaatcc ctcggaagct gcgcctgtca
      301 tcgaattcct gcagcccggt gcatgactaa gctagtaccg gtggggagct gtggaagcag
      361 taggggagct gtggaagcag taggggagct gtggaagcag taggggagct gtggaagcag
      421 taggggagct gtggaagcag taggggagct gtggaagcag tagctacgac gattggtact
      481 ccccagttaa ggtcggctag ctcagttagc ctcccccatc tctcgacgcg gccgctttac
      541 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      601 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      661 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      721 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      781 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtact
//
