LOCUS       sequence-167417-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-296-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    143..277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(180..199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(599..620)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattttatcg atcacgagac tagcctcgag agatctgatc ataatcagcc ataccacatt
       61 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      121 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataagg
      181 caatagcatc acaaatttca caaataaggc atttttttca ctgcattcta gttttggttt
      241 gtccaaactc atcaatgtat cttatcatgt ctggatctca aatccctcgg aagctgcgcc
      301 tgtcatcgaa ttcctgcagc ccggtgcatg actaagctag taccggtgag ggttgggtgg
      361 aggctctccg agggttgggt ggaggctctc cgagggttgg gtggaggctc tccgagggtt
      421 gggtggaggc tctccgaggg ttgggtggag gctctccgag ggttgggtgg aggctctccg
      481 ctacgtcgac cagtactccc cagttaaggt cggctagctc agttagcctc ccccatctct
      541 cgacgcggcc gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      601 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
      661 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      721 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
      781 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
      841 tgtactccag cttgtg
//
