LOCUS       sequence-167418-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-345-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    142..276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(179..198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(598..619)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attttatcga tcacgagact agcctcgaga gatctgatca taatcagcca taccacattt
       61 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
      121 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaggc
      181 aatagcatca caaatttcac aaataaggca tttttttcac tgcattctag ttttggtttg
      241 tccaaactca tcaatgtatc ttatcatgtc tggatctcaa atccctcgga agctgcgcct
      301 gtcatcgaat tcctgcagcc cggtgcatga ctaagctagt accggtgctg actcctagtc
      361 cagggctcgc tgactcctag tccagggctc gctgactcct agtccagggc tcgctgactc
      421 ctagtccagg gctcgctgac tcctagtcca gggctcgctg actcctagtc cagggctcgc
      481 tacgtcgacc agtactcccc agttaaggtc ggctagctca gttagcctcc cccatctctc
      541 gacgcggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      601 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      661 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      721 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      781 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      841 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg a
//
