LOCUS       sequence-167422-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    133..267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(170..189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(589..610)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcacgagac tagcctcgag agatctgatc ataatcagcc ataccacatt tgtagaggtt
       61 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      121 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataagg caatagcatc
      181 acaaatttca caaataaggc atttttttca ctgcattcta gttttggttt gtccaaactc
      241 atcaatgtat cttatcatgt ctggatctca aatccctcgg aagctgcgcc tgtcatcgaa
      301 ttcctgcagc ccggtgcatg actaagctag taccggttgg agagaaaggc agttcctgat
      361 ggagagaaag gcagttcctg atggagagaa aggcagttcc tgatggagag aaaggcagtt
      421 cctgatggag agaaaggcag ttcctgatgg agagaaaggc agttcctgag ctacgtcgac
      481 cagtactccc cagttaaggt cggctagctc agttagcctc ccccatctct cgacgcggcc
      541 gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      601 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      661 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      721 gtcggcgagc tgcac
//
