LOCUS       sequence-167426-        4614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FAM116A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FAM116A_p.R427H
FEATURES             Location/Qualifiers
     source          1..4614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2014..2113)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2130..2149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2131..2149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2154..2170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2154..2170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2601..3392
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3485..4073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3974..3993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4227..4244
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4403..4430)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4522..4608)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggctttgagg ggccctgcgg gcttggggcc cggctctcga aggccgttgg
      241 acgaagcggt ggcaggggcc gagggccgcg aggcgccggc ccttgtggcg gcgggaggcg
      301 cgccagagga cgatgaagag gacgatggcc gtggccgggg cctgctgcgc tgggacagct
      361 tctccgcctg gctgcactgc gtgtgtgtgg tgggcttcga cctggagctg ggccaggccg
      421 tggaggtaat ttatcctcag cattccaaac ttactgacag agaaaaaacc aatatttgct
      481 atttgtcttt tccagattca aattcaggtt gtcttggaga tacccagttt tgttttagat
      541 ttcgacagtc ttctgggagg agggtgtcgc tgcattgtct cctggatcaa tttgacaaag
      601 atttaccagt ttacttaaag aaggatcctg cttattttta tggatatgtg tatttccgac
      661 aagttcgaga taaaactcta aaaagaggct actttcagaa gtccttggtt ttgatcagca
      721 aactacctta tattcatttt tttcacactg tgctcaaaca gatagcacca gagtattttg
      781 aaaagaatga accttatttg gaagcagctt gtaatgatgt tgatcgatgg cctgccccag
      841 tgccagggaa aacattacac ctgccaatca tgggggtggt aatgaaggta cggattccca
      901 catgtcatga caagcctggg acaactcaaa tagtgcagtt aactcagcag gtggacacaa
      961 atatatctgt tattttacct actgttcatg aggtggatat tttcaggtgt ttctgcccag
     1021 ttttccttca tagtcagatg ctctgggagc tggtgctgtt gggggagccc cttgtggtta
     1081 tggcgccatc accatcggaa tcatcagaga ctgtattggc acttgttaac tgtatttctc
     1141 cattaaagta cttcagtgat ttccgacctt atttcactat tcatgatagt gaattcaaag
     1201 aatatactac ccgtacgcaa gctccgccct cagttatatt aggagtaacc aacccttttt
     1261 ttgctaagac actccagcac tggccacaca ttattcgaat aggagacctt aaacctacag
     1321 gtgaaattcc taagcaggtt aaagtgaaaa aactgaagaa tctaaagact ctggattcca
     1381 aacctggagt ttatacttca tataagccat atttaaatag agatgaagag atcataaaac
     1441 aattacagaa gggtgtacaa cagaaacatc cttctgaggc tcaaagtgtt attcttcgac
     1501 gctatttttt ggaactgaca caaagtttca tcattccatt agaaagatat gtggcaagct
     1561 tgatgccttt gcagaaaagt atttccccat ggaagagtcc acctcaatta agacagtttc
     1621 ttccagaaga atttatgaaa acacttgaga aaacaggacc tcagctaacc tctagaataa
     1681 aaggcgattg gattggactt taccggcatt tcctaaagtc tccaaatttt gatggctggt
     1741 ttaagacccg gaggaaggaa atgacccaaa aattggaggc actccatcta gaagctcttt
     1801 gtgaagagga cttacttctc tggatccaga aacacacaga agtagaaaca gtagaccttg
     1861 tcttgaagct gaaaaataag ctgttgcagg ctgatcgaga gcacttacct gtgaaacctg
     1921 acactatgga aaagttacgg acacacatag atgccattat cttagcattg ccagaggact
     1981 tgcaaggcat actgctcaaa acgggcatga catgcccaac tttcttgtac aaagttggca
     2041 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2101 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2161 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2221 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2281 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2341 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2401 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2461 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2521 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2581 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2641 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2701 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2761 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2821 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2881 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2941 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3001 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3061 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3121 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3181 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3241 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3301 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3361 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3421 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3481 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3541 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3601 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3661 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3721 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3781 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3841 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3901 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3961 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4021 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4081 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4141 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4201 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4261 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4321 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4381 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4441 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4501 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4561 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcct
//
