LOCUS       sequence-167440-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the Golgi apparatus (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pmScarlet-H_Giantin_C1
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..730
                     /codon_start=1
                     /product="monomeric red fluorescent protein evolved from a 
                     synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-H
                     /note="more photostable than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     HALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggcagtgat
       61 caaggagttc atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg
      241 ctccagggcc ttcaccaagc accccgccga catccccgac tactataagc agtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac
      361 ccaggacacc tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa
      421 cttccctcct gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga
      481 gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt aagcacgccc tgcgcctgaa
      541 ggacggcggc cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca
      601 gatgcccggc gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta
      661 caccgtggtg gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga
      721 gctgtacaag tccggactca gatctcgagg agaaccgcag caaagct
//
