LOCUS       sequence-167448-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the cytoplasm, but not the nucleus (can be 
            used for colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pmScarlet-H_NES_C1
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(171..189)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     439..458
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggcagt
       61 gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt
      121 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      181 ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta
      241 cggctccagg gccttcacca agcaccccgc cgacatcccc gactactata agcagtcctt
      301 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt
      361 gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac
      421 caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcgtccac
      481 cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagcacg ccctgcgcct
      541 gaaggacggc ggccgctacc tggcggactt caagaccacc tacaaggcca agaagcccgt
      601 gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc acaacgagga
      661 ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cact
//
