LOCUS       sequence-167454-        3975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP2K1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP2K1_p.C121S
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     416..433
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(592..619)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(711..797)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     846..868
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     860..877
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     861..877
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    893..992
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2178..2277)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2294..2313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2295..2313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2318..2334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2318..2334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2765..3556
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3649..3975,1..262)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
       61 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      301 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      361 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      421 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      481 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      541 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
      601 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
      661 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
      721 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
      781 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
      841 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat
      901 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
      961 tataatgcca actttgtaca aaaaagttgg caccatgccc aagaagaagc cgacgcccat
     1021 ccagctgaac ccggcccccg acggctctgc agttaacggg accagctctg cggagaccaa
     1081 cttggaggcc ttgcagaaga agctggagga gctagagctt gatgagcagc agcgaaagcg
     1141 ccttgaggcc tttcttaccc agaagcagaa ggtgggagaa ctgaaggatg acgactttga
     1201 gaagatcagt gagctggggg ctggcaatgg cggtgtggtg ttcaaggtct cccacaagcc
     1261 ttctggcctg gtcatggcca gaaagctaat tcatctggag atcaaacccg caatccggaa
     1321 ccagatcata agggagctgc aggttctgca tgagagcaac tctccgtaca tcgtgggctt
     1381 ctatggtgcg ttctacagcg atggcgagat cagtatctgc atggagcaca tggatggagg
     1441 ttctctggat caagtcctga agaaagctgg aagaattcct gaacaaattt taggaaaagt
     1501 tagcattgct gtaataaaag gcctgacata tctgagggag aagcacaaga tcatgcacag
     1561 agatgtcaag ccctccaaca tcctagtcaa ctcccgtggg gagatcaagc tctgtgactt
     1621 tggggtcagc gggcagctca tcgactccat ggccaactcc ttcgtgggca caaggtccta
     1681 catgtcgcca gaaagactcc aggggactca ttactctgtg cagtcagaca tctggagcat
     1741 gggactgtct ctggtagaga tggcggttgg gaggtatccc atccctcctc cagatgccaa
     1801 ggagctggag ctgatgtttg ggtgccaggt ggaaggagat gcggctgaga ccccacccag
     1861 gccaaggacc cccgggaggc cccttagctc atacggaatg gacagccgac ctcccatggc
     1921 aaattttgag ttgttggatt acatagtcaa cgagcctcct ccaaaactgc ccagtggagt
     1981 gttcagtctg gaatttcaag attttgtgaa taaatgctta ataaaaaacc ccgcagagag
     2041 agcagatttg aagcaactca tggttcatgc ttttatcaag agatctgatg ctgaggaagt
     2101 ggattttgca ggttggctct gctccaccat cggccttaac cagcccagca caccaaccca
     2161 tgctgctggc gtctgattgc caactttctt gtacaaagtt ggcattataa gaaagcattg
     2221 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2281 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2341 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2401 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     2461 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2521 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2581 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     2641 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2701 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2761 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2821 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2881 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     2941 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     3001 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3061 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3121 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3181 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3241 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3301 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3361 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3421 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3481 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3541 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     3601 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3661 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3721 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3781 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3841 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3901 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3961 gggctgaacg ggggg
//
