LOCUS       sequence-167457-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil  peptide SYNZIP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP1
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(251..350)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(367..386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(368..386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(391..407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(391..407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(509..528)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(587..606)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 catcaccatc accatcacca tggtgctgca ggtacaagtt tgtacaaaaa agcaggctcc
       61 gcggccgccc ccttcaccat gggatccaac ctggttgcgc agctcgaaaa cgaagttgcg
      121 tctctggaaa atgagaacga aaccctgaag aaaaagaacc tgcacaaaaa agacctgatc
      181 gcgtacctgg agaaagaaat cgcgaatctg cgtaagaaaa tcgaagaatg ataaaagggt
      241 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
      301 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
      361 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
      421 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
      481 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
      541 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
      601 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
      661 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
      721 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
      781 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
      841 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
      901 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
      961 gctcaggcgc aatcacgaat gaataacggt t
//
