LOCUS       sequence-167463-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression and/or retrovirus preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pQCXIP-Laforin Myc K320A
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             626..655
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             671..688
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cactggacac accaatgaaa tgaagcacac aacagacttc tattttaata ttgcaggcca
       61 ccaagccatg cattattcaa gaattctacc aaatatctgg ctgggtagct gccctcgtca
      121 ggtggaacat gtaaccatca aactgaagca tgaattgggg attacagctg taatgaattt
      181 ccagactgaa tgggatattg tacagaattc ctcaggctgt aaccgctacc cagagcccat
      241 gactccagac actatgatta aactatatag ggaagaaggc ttggcctaca tctggatgcc
      301 aacaccagat atgagcaccg aaggccgagt acagatgctg ccccaggcgg tgtgcctgct
      361 gcatgcgctg ctggagaagg gacacatcgt gtacgtgcac tgcaacgctg gggtgggccg
      421 ctccaccgcg gctgtctgcg gctggctcca gtatgtgatg ggctggaatc tgaggaaggt
      481 gcagtatttc ctcatggcca agaggccggc tgtctacatt gacgaagagg ccttggcccg
      541 ggcacaagaa gattttttcc aggcatttgg gaaggttcgt tcttctgtgt gtagcctgta
      601 ctcgagtcta gagggcccgc ggttcgaaca aaaactcatc tcagaagagg atctgaatat
      661 gcataccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
      721 gcctttctag tt
//
