LOCUS       sequence-167464-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression and/or retrovirus preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pQCXIP-Laforin Myc 241X
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             355..384
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             400..417
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(440..457)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    446..670
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tggattgagg ccactggaca caccaatgaa atgaagcaca caacagactt ctattttaat
       61 attgcaggcc accaagccat gcattattca agaattctac caaatatctg gctgggtagc
      121 tgccctcgtc aggtggaaca tgtaaccatc aaactgaagc atgaattggg gattacagct
      181 gtaatgaatt tccagactga atgggatatt gtacagaatt cctcaggctg taaccgctac
      241 ccagagccca tgactccaga cactatgatt aaactatata gggaagaagg cttggcctac
      301 atctggatgc caacaccaga tatgagcacc gaaggcctag agggcccgcg gttcgaacaa
      361 aaactcatct cagaagagga tctgaatatg cataccggtc atcatcacca tcaccattga
      421 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      481 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      541 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
      601 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
      661 ggctctatgg cggatccgga attccgcc
//
