LOCUS       sequence-167472-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in
            E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pC3R112C/T180C
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    81..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             120..142
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             161..178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             188..205
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
      121 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
      181 cagcggcctg gtgccgcgcg gcagccatat gactaccgaa accatccagt ctaacgcgaa
      241 cctggctccg ctgccgccgc atgtgccgga acacctggtg ttcgacttcg atatgtacaa
      301 cccgtctaac ctgtctgcgg gcgttcagga agcgtgggcg gtgctgcagg aatccaacgt
      361 tccggatctg gtgtggaccc gctgcaacgg cggtcactgg atcgcgaccc gtggccagct
      421 gattcgtgaa gcatacgaag attaccgtca tttctcctct gaatgcccgt tcatcccgcg
      481 tgaggctggt gaggcgtacg atttcattcc gactagcatg gacccgccgg agcagcgcca
      541 gtttcgtgcg ctggcgaacc aggttgttgg tatgccggtt gtggataaac tggagaaccg
      601 tatccaggag ctggcgtgct ctctgattga gtctctgcgt ccgcagggtc agtgcaactt
      661 caccgaagac tacgcggaac cgtttccgat ccgtattttc atgctgctgg cgggtctgcc
      721 ggaggaagac attccgcacc tgaagtatct gaccgaccag atgactcgcc cggatggctc
      781 tatgaccttc gcggaggcga aagaagcgct gtacgactac ctgatcccga tcatcgaaca
      841 gcgccgtcag aagccgggta ccgacgcgat ctctatcgtt gcgaacggtc aggttaacgg
      901 tcgtccgatt acctccgatg aagcgaaacg tatgtgcggt ctgctgctgg tgggtggtct
      961 gga
//
