LOCUS       sequence-167473-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR 
            of the queC gene from B. subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(248..264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(248..264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(261..283)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    272..288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(296..302,303..320,321..326))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    341..362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(479..496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(730..749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcttgcatgc ctgcaggtcg actctagagg atcccaaaac acttgatact gtatgagcat
       61 acagtataat tgcttcggta ccgtgcgata tgcgggagag gttctagcta caccctctat
      121 aaaaaactaa ggacgagctg tatccttgga tacggccttt tttatgtttt tctagagcac
      181 cttccgaaaa aaggtgtttt tttgcgtgaa ttagctgtag cgatgtctct cgcgaattcg
      241 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      301 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      361 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      421 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      481 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      541 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      601 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      661 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      721 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      781 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      841 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      901 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      961 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
//
