LOCUS       sequence-167475-        2869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR 
            of the queC gene from B. subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR
FEATURES             Location/Qualifiers
     source          1..2869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(661..683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    672..688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(696..702,703..720,721..726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    741..762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(879..896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1050..1638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1130..1149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1809..2600,2601..2669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2432..2451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2670..2774)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2842..2860
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atcccaaaac acttgatact gtatgagcat acagtataat tgcttcggta
      481 ccgtgcgata tgcgggagag gttctagcta caccctctat aaaaaactaa ggacgagctg
      541 tatccttgga tacggccttt tttatgtttt tctagagcac cttccgaaaa aaggtgtttt
      601 tttgcgtgaa ttagctgtag cgatgtctct cgcgaattcg taatcatgtc atagctgttt
      661 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      721 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      781 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      841 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     1981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2821 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
