LOCUS       sequence-167478-        5211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express tiam-1 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Zheng C, Diaz-Cuadros M, Chalfie M
  TITLE     GEFs and Rac GTPases control directional specificity of neurite 
            extension along the anterior-posterior axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6973-8. doi: 
            10.1073/pnas.1607179113. Epub 2016 Jun 6.
  PUBMED    27274054
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1607179113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: "6973-8"
COMMENT     Sequence Label: TU#1412_tiam-1_cDNA
FEATURES             Location/Qualifiers
     source          1..5211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3452..3471)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3453..3471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3476..3492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3476..3492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3594..3613)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3605..4414
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3672..3691)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4561..5149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5050..5069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta atgggctcac gcctctcatg ttcttgctca caagccgaat gctggacgaa
      721 tgatgaatct acattgagaa ttgacgcgga gttgtttgag ttatcacagg acggacaaaa
      781 gtgggataag cttgacggaa ggctggccaa cgtacgtgta ttcaacgcat tcgacgactc
      841 ttccccacgt cttcttgcca catcatcatc cggtcaagtt cttctggaca ctctaattcc
      901 aatgggagaa aaggttcata aagtcagcga cttttttgtg tatttgaaga cggaaggaag
      961 aacaattgga ttcaatacgt taagctcgag ggacacttcc ttactcattt ctcaggcgac
     1021 aaatacaact gccttcaata tgtttcaaca ttcattctca gtttccgtat ccagaattgc
     1081 tacatttgaa ggtgattcta ttcaaccttg tgatgagcag ccagaaacgt caaatgtctc
     1141 actaaatccg ttaaatatgg ttttcaacta caaaggatca cctgttgaga ttgatttgct
     1201 ggaatgtttc gcgtgctcat cagaagctga aaactgtgtc gacatagcac atgccaacaa
     1261 catatttcgt gtaattgtgt cagcacatgc tcatcttttt cttattcaag ctctgaatgt
     1321 atcatcactt cttcttgctc gaaccacctc gaatctagtt gccatcaatt atttggctcg
     1381 gcaaaatgat aaaattcaac aaaaattgat tgctctgttg gaaacatcag aatcaagtga
     1441 gaatgcgtcg aaggaagata ttcgacaaat gcttcttctg aagaatgtag tagtgacaca
     1501 gagattgaac tcacttcaac agagagcact accgggaatt gagcttatcc aacacgatat
     1561 ctcacctcaa attgatcaga agctactcga aaagtgtcga cttccatcac tgtttttata
     1621 tcattttaat atgaattcgt ttcgaatatg gcgaaaacca atggaaacac gtgacatgaa
     1681 cacaatggga tcatcaatgg catccaccag tcaataccat cagattccga ttcaaatcca
     1741 gaatcagaat ccgttaccgt ctcaacagat tcagccacaa caacaacaac aataccatcc
     1801 accagatcat gtgacaactc ataatggtac aactacgttt catactacga atcccacaaa
     1861 accaagactt tgtgaaggat cgttggctcc tggatgctca ttggccgagg catccgaaca
     1921 gagaaaacga gagatggcgg agcagaaaaa agagttggca ggcccatctt ccgcagagga
     1981 ttacttcgtc agaaagacaa atggacgtct tggactcact atatacgcgc ataacgatga
     2041 tggggtcatc agggctgagg ttcgtggagt gacgtcattt gctccgagat gtgctcaagt
     2101 tggagattcg gttgtagcgg tagacagtga actcatatca tcagttagaa acgcttcgga
     2161 tgttgagaaa cttttgagaa tcggaaaagt aatacattta cgaagaaaga ctcctctaac
     2221 tccaagagca ccgtctcaac tcgacgcaac taatgtcaat aagaaaacgg gctgtgcaaa
     2281 gttggcgatg gctctgcaag aattgttggt cactgagaag aaatatgtca gcgatcttcg
     2341 agagatgaat gaagtttttc tgtgtatgag acaagtccga gatattatga atgcagcaat
     2401 acggttgtat aaaatgcaaa catcgtttgt ggacagtatt gaggaagcca ttggagacat
     2461 gagtagacag gatatttctg ttgctcagat tagggattct gtaatgcgag tgtgtgcagt
     2521 attcatcaac aaatgttctg atttcaaaat ctatgcagaa tatgcagccg gttaccatct
     2581 cctccaacat gagatcaaaa acaaaaaaga gcttctatca aaattggaag ctgtaaacag
     2641 tacaagagag caacattgtt cttgggaatc tcggatgatt aaaccagtcc agagaattgt
     2701 acaatatcca cttttactga aaaacattgc cgatgcactt ccgaaggatg cacgagagag
     2761 ggttcaagtg gaggcagcat tgcaaaaaat gcagacgtct gcagaatatg tgaatgaaat
     2821 gcaaaggctt catgaagatt atgcacaata tatggagact gttaggaaag ctaatgagcc
     2881 aatgttggct gaaaagagtc ttcgcctcga cactcgagag cttctagttt ttgcacacat
     2941 aaaatggaga gatgctccag cggagcacta cgttgtgttc gtgtttcact ctttaatact
     3001 gctccttcca tcgtatgcta ggaaagagat taagatgaaa tggactcgtg tcctcccaat
     3061 aaatgaagtt gatatcaacg aaatgccaaa tgatgcatta aatctaaaac tttatcatgc
     3121 tgcttttgaa ggaccaaatg gacaattaag tcattccaat ccaaatacag tctacaatat
     3181 tgaatgttgt caatctcaac tgaaacagca tcttatcaaa aatatcaaaa aagctcgaac
     3241 tttattctct cgagaatcac atcgtccatt aagtggatct agtcaatcgg atggtggtta
     3301 tgtgtcagaa gtctctaagg aacatcgaaa ttcacaccca gctttcttgt agaaagttgg
     3361 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     3421 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     3481 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3541 taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     3601 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     3661 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     3721 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     3781 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     3841 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     3901 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     3961 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     4021 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     4081 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     4141 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     4201 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     4261 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     4321 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     4381 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     4441 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     4501 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4561 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4621 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4681 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4741 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4801 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4861 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4921 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4981 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5041 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5101 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5161 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
