LOCUS       sequence-16748-e        1277 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4018
FEATURES             Location/Qualifiers
     source          1..1277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             254..274
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             296..316
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             338..358
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             380..400
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(443..471)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnnnttacat gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg
       61 ctgancatca ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg
      121 cctgcaggtc gactctagag gatccccggg attggccaaa ggacccaaag gtatgtttcg
      181 aatgatacta acataacata gaacattttc aggaggaccc ttgagggtac cgagctcaga
      241 aaaaatgact gctccaaaga agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa
      301 gaagaagcgt aaggtaccgg cagaaaaaat gactgctcca aagaagaagc gtaaggtacc
      361 ggcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggtagaaa aaatgagtaa
      421 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      481 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      541 ccttaaattt atttgcacta ctggaaaact acctgttcca tgggtaagtt taaacatata
      601 tatactaact aaccctgatt atttaaattt tcagccaaca cttgtcacta ctttctgtta
      661 tggtgttcaa tgcttctcga gatacccaga tcatatgaaa cggcatgact ttttcaagag
      721 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      781 caagacacgt aagtttaaac agttcggtac taactaacca tacatattta aattttcagg
      841 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      901 ttttaaagaa gatggaaaca ttcttggaca ccaattggaa tacnactata actcacacaa
      961 tgtatacctc ntggcagaca acnaaagaat ggaatccaag ttgtaagttt aaacntgatt
     1021 ttnctaacta nctatctgat ttaantttcn naactncnaa attagaccca cnttgaaaat
     1081 ggaagcgttc actngccnac ccttntcacc aaatactcca ttggngatgg ncctgtcntt
     1141 tnccnnaaac cnttnctgtc cccccantct gccntttcna anatcccncc naaaaanaac
     1201 ccccnggccc tccttgggtt ngaaccnctc cgggaannnc ccngggcngg gggaactncn
     1261 aaaancctcn tnaantc
//
