LOCUS       sequence-16749-e        1326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4019
FEATURES             Location/Qualifiers
     source          1..1326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             257..277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             299..319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             341..361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             383..403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(446..474)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 anngttnact gatcttctaa ctaactattc tcatttaaat tttcagagct taaaaatggc
       61 tgacctcnct cacaacgatg gatacgctaa caacttggaa atgaaataag cttgcatgcc
      121 tgcaggtcga ctctagagga tccccgggat tggccaaagg acccaaaggt atgtttcgaa
      181 tgatactaac ataacataga acattttcag gaggaccctt gcttggaggg taccgagctc
      241 agaaaaaatg actgctccaa agaagaagcg taaggtaccg gcagaaaaaa tgactgctcc
      301 aaagaagaag cgtaaggtac cggcagaaaa aatgactgct ccaaagaaga agcgtaaggt
      361 accggcagaa aaaatgactg ctccaaagaa gaagcgtaag gtaccggtag aaaaaatgag
      421 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      481 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      541 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatgggtaa gtttaaacat
      601 atatatacta actaaccctg attatttaaa ttttcagcca acacttgtca ctactttctg
      661 ttatggtgtt caatgcttct cgagataccc agatcatatg aaacggcatg actttttcaa
      721 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      781 ctacaagaca cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
      841 aggtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaggtatt
      901 gattttaaag aagatggaac attcttggac acaattggaa tacanctata actcacacat
      961 gtatacntca tggcaaacaa ccaaaganng gaatcaagtt gtaagtttaa cntgatttac
     1021 tactaactat ctgattnaat ttccgaactc caattaaacc cacntgagan gagcgtcact
     1081 ncaacntttc acaaatctcc atggngangn ctgtctttac nanacntact gtccnatngc
     1141 cttncaaatc naaaaaaaaa ccntggcctt tgattgacnc tcggatccng gcggggacta
     1201 caancttccn aatnanttac ttccncnaan aacncctttt atttaaatcc cgggccnccc
     1261 nntnccntgg gggnaaaagg ccnnccnaat tncgttcncc nncaaatttt tncncccccn
     1321 cccccc
//
