LOCUS       sequence-167498-        9155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kv10.1 fused mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Chen Y, Sanchez A, Rubio ME, Kohl T, Pardo LA, Stuhmer W
  TITLE     Functional K(v)10.1 channels localize to the inner nuclear membrane.
  JOURNAL   PLoS One. 2011 May 3;6(5):e19257. doi: 10.1371/journal.pone.0019257.
  PUBMED    21559285
REFERENCE   2  (bases 1 to 9155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-05-3"; volume: "6(5):e19257. doi"; pages: " 
            10.1371/journal.pone.0019257"
COMMENT     Sequence Label: pcDNAKv10.1mVenus
FEATURES             Location/Qualifiers
     source          1..9155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     5'UTR           889..931
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             complement(2673..2687)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     regulatory      3890..3899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3896..3898,3899..3901,3902..4615)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3941..3962)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4202..4221)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4549..4570
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4707..4725)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4708..4725)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4745..4762)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4751..4975
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5021..5449
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5108..5127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5318..5339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5458..5478)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5463..5793
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5644..5779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5706..5725
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5860..6654
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5914..5933)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6524..6543
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6828..6949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6865..6884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6919..6938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6998..7014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6998..7014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7011..7033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7022..7038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7046..7052,7053..7070,7071..7076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7091..7112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7229..7246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7400..7988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7480..7499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8159..8950,8951..9019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8782..8801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9020..9124)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagaatt
      841 aattcagatc tggtaccggg ccccccctcg agctcggtac ccagcttgct tgttcttttt
      901 gcagaagctc agaataaacg ctcaactttg gcagatcaat tccccccacc gcggtggcgg
      961 ccggaattcc gggcccgccg gaccccgagc tgctgggagg atgaccatgg ctgggggcag
     1021 gaggggacta gtggcccctc aaaacacgtt tctggagaat attgttcggc ggtccaatga
     1081 tactaatttt gtgttgggga atgctcagat agtggactgg cctattgtgt acagcaatga
     1141 tggattttgc aagctgtctg gctatcacag ggcagaagtg atgcaaaaaa gcagcacctg
     1201 cagttttatg tatggggagc tgactgataa agacacgatt gaaaaagtgc ggcaaacatt
     1261 tgagaactat gagatgaatt cctttgaaat tctgatgtac aagaagaaca ggacacctgt
     1321 gtggttcttt gtgaaaattg ctccaattcg aaacgaacag gataaagtgg ttttatttct
     1381 ttgcactttc agtgacataa cagctttcaa acagccaatt gaggatgatt catgtaaagg
     1441 ctgggggaag tttgctcggc tgacaagagc actgacaagc agcaggggtg tcctgcagca
     1501 gctggctcca agcgtgcaaa aaggcgagaa tgtccacaag cactcccgcc tggcagaggt
     1561 cctacagctg ggctcagaca tccttcccca gtacaagcaa gaggcaccaa agactccccc
     1621 tcacatcatc ttacattatt gtgtttttaa gaccacgtgg gattggatca tcttgatctt
     1681 gaccttctat acagccatct tggtccctta taatgtctcc ttcaaaacca ggcagaataa
     1741 tgtggcctgg ctggttgttg atagcatcgt ggatgttatc tttttggtgg acattgtgct
     1801 caattttcat accacctttg ttggaccagc aggggaggtg atttctgacc ccaaacttat
     1861 ccgcatgaac tacctgaaga cgtggtttgt gattgacctt ctgtcctgtt tgccatatga
     1921 tgtcatcaac gcttttgaga acgtggatga gggcatcagc agcctgttca gctctctaaa
     1981 agttgtccgg ctgctccgtc ttgggcgagt ggcccgtaag ctggaccact acattgaata
     2041 tggagctgct gtgctggtcc tgctggtgtg tgtgtttggg ctggctgcac actggatggc
     2101 ctgcatctgg tacagcattg gggactatga gatctttgac gaggacacca agacaatccg
     2161 caacaacagc tggctgtacc aactagcgat ggacattggc accccttacc agtttaatgg
     2221 gtctggctca gggaagtggg aaggtggtcc cagcaagaat tctgtctaca tctcctcgtt
     2281 gtatttcaca atgaccagcc tcaccagtgt gggctttggg aacatcgccc catccacaga
     2341 cattgagaag atctttgcag tggccatcat gatgattggc tcacttctct atgccaccat
     2401 cttcgggaat gtgacgacta ttttccaaca gatgtatgcc aacaccaaca gataccatga
     2461 gatgctcaac agtgttcggg acttcctgaa gctctaccag gtgccaaaag gattgagtga
     2521 gcgagtaatg gattatattg tgtccacttg gtccatgtcc agaggcattg acacagagaa
     2581 ggtcctgcag atctgcccca aggacatgag agccgacatc tgcgtgcacc tgaaccgcaa
     2641 ggtgttcaag gagcacccgg ccttccggct ggccagtgat ggctgcctcc gggcactggc
     2701 catggagttc cagacggtgc actgtgcccc aggggacctc atctaccatg caggagagag
     2761 cgttgacagc ctctgctttg tggtttctgg ctccctggag gtgatccaag atgatgaggt
     2821 ggtggccatt ctaggaaaag gagacgtgtt tggagatgtg ttctggaagg aagccaccct
     2881 tgcccagtcc tgtgccaatg ttagggcctt gacctactgt gatctgcatg tgatcaagcg
     2941 ggatgccctg cagaaagtgc tggaattcta cacggccttc tcccattcct tctcccggaa
     3001 cctgattctg acgtacaact tgaggaagag gattgtgttc cggaagatca gcgatgtgaa
     3061 acgtgaagag gaagaacgca tgaaacgaaa gaatgaggcc cccctgatct tgcccccgga
     3121 ccaccctgtc cggcgcctct tccagagatt ccgacagcag aaagaggcca ggctggcagc
     3181 tgagagaggg ggccgggacc tggatgacct agatgtggag aagggcaatg tccttacaga
     3241 gcatgcctcc gccaaccaca gcctcgtgaa ggccagcgtg gtcaccgtgc gtgagagtcc
     3301 tgccacgccc gtatccttcc aggcagcctc cacctccggg gtgccagacc acgcaaagct
     3361 acaggcgcca gggtccgagt gcctgggccc caaggggggc gggggcgatt gtgccaagcg
     3421 caaaagctgg gcccgcttca aagatgcttg cgggaagagt gaggactgga acaaggtgtc
     3481 caaggctgag tcgatggaga cacttcccga gaggacaaaa gcgtcaggcg aggccacact
     3541 gaagaagaca gactcgtgtg acagtggcat caccaagagc gacttgcgcc tggacaacgt
     3601 gggtgaggcc aggagtcccc aggatcggag tcccatcctg gcagaggtca agcattcgtt
     3661 ctaccccatc cctgagcaga cgctgcaggc cacagtcctg gaggtgaggc acgagctgaa
     3721 ggaggacatc aaggccttaa acgccaaaat gaccaatatt gagaaacagc tctctgagat
     3781 actcaggata ttaacttcca gaagatcctc tcagtctcct caggagttgt ttgaaatatc
     3841 gaggccacag tccccagaat cagagagaga catttttgga gccagcgcgg ccgccatggt
     3901 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3961 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4021 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4081 gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     4141 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4201 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     4261 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     4321 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     4381 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     4441 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4501 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4561 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaggatc
     4621 cactagtaac ggccgccagt gtgctggaat tctgcagata tccatcacac tggcggccgc
     4681 tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
     4741 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4801 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4861 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4921 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4981 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     5041 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5101 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5161 caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5221 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5281 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5341 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg
     5401 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     5461 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa
     5521 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5581 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5641 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5701 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5761 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5821 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5881 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5941 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6001 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     6061 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6121 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6181 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6241 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6301 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6361 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     6421 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     6481 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     6541 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     6601 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     6661 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6721 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6781 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6961 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7021 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7081 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7141 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7201 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7261 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7321 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7381 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7441 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7501 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7561 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     7621 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7681 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7741 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7801 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7861 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7921 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7981 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8041 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8101 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8161 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8221 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8281 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8341 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8401 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8461 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8521 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8581 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8641 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8701 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8761 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8821 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8881 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8941 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9001 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9061 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9121 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
