LOCUS       sequence-16750-e        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4020
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             248..268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             290..310
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             332..352
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             374..394
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(437..465)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gttactgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
       61 aatcactcac aacgatggat acgctaacaa cttggaaatg aaataagctt gcatgcctgc
      121 aggtcgacta gaggatcccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata
      181 ctaacataac atagaacatt ttcaggagga cccttggagg gtaccgagct cagaaaaaat
      241 gactgctcca aagaagaagc gtaaggtacc ggcagaaaaa atgactgctc caaagaagaa
      301 gcgtaaggta ccggcagaaa aaatgactgc tccaaagaag aagcgtaagg taccggcaga
      361 aaaaatgact gctccaaaga agaagcgtaa ggtaccggta gaaaaaatga gtaaaggaga
      421 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      481 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      541 atttatttgc actactggaa aactacctgt tccatgggta agtttaaaca tatatatact
      601 aactaaccct gattatttaa attttcagcc aacacttgtc actactttct gttatggtgt
      661 tcaatgcttc tcgagatacc cagatcatat gaaacggcat gactttttca agagtgccat
      721 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      781 acgtaagttt aaacagttcg gtactaacta accatacata tttaaatttt caggtgctga
      841 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
      901 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac cnatgtatac
      961 atcntggcag acaaccnaaa gaatggaatc caagttgtaa gtttaacntg atttnctaac
     1021 tanctatctg atttaatttt cnaactccaa attaacccac nttgaantgg agcgttcact
     1081 accnaccttn tcacaaattc ccnttgggan gccngtcctt tnccnaaacn tnctgtcccn
     1141 atngnccttt caanatccnn caaananaac cntggcctnt gatttaaccc gcnggatacc
     1201 cggcgggana ctnccaaacc tccnantggn ttacttccnc ncccacccct ttatttnaat
     1261 ccggggcggn cccttnnntn ggggaaaaag gccccccaaa tnacnnncnc nccaattntt
     1321 taccccngnn aaaaaattcn nccgtt
//
