LOCUS       sequence-167500-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pVR2 but with G to U and A to C mutations following the riboswitch 
            aptamer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2-m4
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(262..279)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(262..278)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(271..293)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(487..506)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     606..628
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(666..684)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        752..856
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attacgaatt cgcgagagac atcgctacag ctaattcacg caaaaaaaca ccttttttcg
       61 gaaggtgctc tagaaaaaca taaaaaaggc cgtatccaag gagacagatc gtccttagtt
      121 ttttatagag ggtgtagcta gaacctctcc cgcatatcgc acggaacgca gagcggtacc
      181 gaagcaatta tactgtatgc tcatacagta tcaagtgttt tgggatcctc tagagtcgac
      241 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      301 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      361 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      421 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      481 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      541 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      601 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
      661 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      721 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      961 agatcagttg ggtgcacgag tgggttacat cgaactgg
//
