LOCUS       sequence-167507-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pVR2 but with an insertion of 1 U immediately following the 
            riboswitch aptamer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2-m5
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(255..272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(255..271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(480..499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     599..621
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(659..677)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        745..849
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttcgcgagag acatcgctac agctaattca cgcaaaaaaa cacctttttt cggaaggtgc
       61 tctagaaaaa cataaaaaag gccgtatcca aggatacagc tcgtccatta gttttttata
      121 gagggtgtag ctagaacctc tcccgcatat cgcacggaac gcagagcggt accgaagcaa
      181 ttatactgta tgctcataca gtatcaagtg ttttgggatc ctctagagtc gacctgcagg
      241 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      301 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      361 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      421 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      481 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      541 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      601 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
      661 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      721 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      781 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      841 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      901 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tga
//
