LOCUS       sequence-167508-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pVR2 but with an insertion of 2 U's immediately following the 
            riboswitch aptamer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2-m6
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(257..274)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(257..273)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(266..288)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(482..501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     601..623
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(661..679)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        747..851
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attcgcgaga gacatcgcta cagctaattc acgcaaaaaa acaccttttt tcggaaggtg
       61 ctctagaaaa acataaaaaa ggccgtatcc aaggatacag ctcgtccaat tagtttttta
      121 tagagggtgt agctagaacc tctcccgcat atcgcacgga acgcagagcg gtaccgaagc
      181 aattatactg tatgctcata cagtatcaag tgttttggga tcctctagag tcgacctgca
      241 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      301 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      361 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
      421 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
      481 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      541 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      601 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      661 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      841 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaa
//
