LOCUS       sequence-167517-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pVR2 but with two G to U mutations immediately following the 
            riboswitch aptamer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2-m8
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(258..275)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(258..274)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(267..289)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(483..502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     602..624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(662..680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        748..852
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaattcgcg agagacatcg ctacagctaa ttcacgcaaa aaaacacctt ttttcggaag
       61 gtgctctaga aaaacataaa aaaggccgta tccaaggata cagctcgtaa ttagtttttt
      121 atagagggtg tagctagaac ctctcccgca tatcgcacgg aacgcagagc ggtaccgaag
      181 caattatact gtatgctcat acagtatcaa gtgttttggg accctctaga gtcgacctgc
      241 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      301 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      361 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
      421 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
      481 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
      541 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
      601 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
      661 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      721 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      781 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      841 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      901 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
//
