LOCUS       sequence-167520-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 in vitro transcription template for Bas mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Chatterjee S, Chauvier A, Dandpat SS, Artsimovitch I, Walter NG
  TITLE     A translational riboswitch coordinates nascent 
            transcription-translation coupling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Apr 20;118(16). pii: 2023426118. doi:
            10.1073/pnas.2023426118.
  PUBMED    33850018
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-04-20"; volume: "118(16). pii: 
            2023426118. doi"; pages: " 10.1073/pnas.2023426118"
COMMENT     Sequence Label: pUC19_BAS1509
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(658..674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(658..674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(671..693)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    682..698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(706..712,713..730,731..736))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    751..772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(889..906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agctcggtac ccggggatcc tctagataat acgactcact atagggtgtt gcttaaaaaa
       61 cgaataacgt ggttcgaaac catcccacgt aaaaaaacta aggagatttt gtcgtgaata
      121 ttagaacatt agtcggtaat ggtattttag cggcattata tattgctgtt tctatgctta
      181 ttcagccatt tggctttacg aatgtacagt ttcgtatttc agagatgttt aatcatctcg
      241 ttgtatttaa taagaaagca atttacggaa ttgtattagg tgtattttta acgaatctct
      301 ttttctcacc tatgattgct tacgatttag tatttggagt agggcaatct attcttgcat
      361 tagttgcaac cattatttct atgcgattca ttaaaggtgt ttgggctcgt atgattttta
      421 atacagttat ctttacaatt acaatgttta tgattgcaat tgaacttcat cttgcatttg
      481 atttaccatt tatgttgact tggttaacat gtgcagtcgg tgaatttgtt gtcatggcca
      541 ttggtatgcc tgtaatgtac tggattaata aacgagtaca atttgaaaga tttatgtaat
      601 agatgaaaga gctattccta tagggatagc tcttttttaa agcttggcgt aatcatggtc
      661 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      721 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      781 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      841 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      961 acggttatcc acagaatcag gg
//
