LOCUS       sequence-167522-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 in vitro transcription template for Bas mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Chatterjee S, Chauvier A, Dandpat SS, Artsimovitch I, Walter NG
  TITLE     A translational riboswitch coordinates nascent 
            transcription-translation coupling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Apr 20;118(16). pii: 2023426118. doi:
            10.1073/pnas.2023426118.
  PUBMED    33850018
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-04-20"; volume: "118(16). pii: 
            2023426118. doi"; pages: " 10.1073/pnas.2023426118"
COMMENT     Sequence Label: pUC19_BAS1509
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(607..626)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(608..626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(660..677)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(660..676)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..691)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(885..904)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttacgccaag ctttaaaaaa gagctatccc tataggaata gctctttcat ctattacata
       61 aatctttcaa attgtactcg tttattaatc cagtacatta caggcatacc aatggccatg
      121 acaacaaatt caccgactgc acatgttaac caagtcaaca taaatggtaa atcaaatgca
      181 agatgaagtt caattgcaat cataaacatt gtaattgtaa agataactgt attaaaaatc
      241 atacgagccc aaacaccttt aatgaatcgc atagaaataa tggttgcaac taatgcaaga
      301 atagattgcc ctactccaaa tactaaatcg taagcaatca taggtgagaa aaagagattc
      361 gttaaaaata cacctaatac aattccgtaa attgctttct tattaaatac aacgagatga
      421 ttaaacatct ctgaaatacg aaactgtaca ttcgtaaagc caaatggctg aataagcata
      481 gaaacagcaa tatataatgc cgctaaaata ccattaccga ctaatgttct aatattcacg
      541 acaaaatctc cttagttttt ttacgtggga tggtttcgaa ccacgttatt cgttttttaa
      601 gcaacaccct atagtgagtc gtattatcta gaggatcccc gggtaccgag ctcgaattca
      661 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      721 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      781 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
      841 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
      901 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
      961 tgtctgctcc cggcatccgc ttacagacaa gctg
//
