LOCUS       sequence-167526-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SpCas9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Duan Y, Ma G, Huang X, D'Amore PA, Zhang F, Lei H
  TITLE     The CRISPR/Cas9-created MDM2 T309G enhances vitreous-induced 
            expression of MDM2 and proliferation and survival of cells.
  JOURNAL   J Biol Chem. 2016 May 31. pii: jbc.M116.729467.
  PUBMED    27246850
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 May 31. pii: jbc.M116.729467."
COMMENT     Sequence Label: pAAV-RSV-SpCas9
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(308..328)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(332..358)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(374..599)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gtctgtggaa gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat
       61 agcagatccg gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg
      121 cctcggctgt ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt
      181 gccggtcggt gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga
      241 tcacggccca gcccacagag ttggtgccga tgtccaggcc gatgctgtac ttcttgtcgg
      301 acgcttcgac cttgcgcttt ttcttcggcg aagcgtaatc tggaacatcg tatgggtaca
      361 tggtggcacc ggttttatgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg
      421 caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta ataaggcacg
      481 atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc cttgtaaggc
      541 atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat aagactacat
      601 ttctagaggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgc
//
