LOCUS       sequence-167528-         424 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SpCas9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Duan Y, Ma G, Huang X, D'Amore PA, Zhang F, Lei H
  TITLE     The CRISPR/Cas9-created MDM2 T309G enhances vitreous-induced 
            expression of MDM2 and proliferation and survival of cells.
  JOURNAL   J Biol Chem. 2016 May 31. pii: jbc.M116.729467.
  PUBMED    27246850
REFERENCE   2  (bases 1 to 424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 May 31. pii: jbc.M116.729467."
COMMENT     Sequence Label: pAAV-RSV-SpCas9
FEATURES             Location/Qualifiers
     source          1..424
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             287..307
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    326..374
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
       61 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
      121 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
      181 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
      241 cctgtacgag acacggatcg acctgtctca gctgggaggc gacagcccca agaagaagag
      301 aaaggtggag gccagctaag aattcaataa aagatcttta ttttcattag atctgtgtgt
      361 tggttttttg tgtgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
      421 gcgc
//
