LOCUS       sequence-167530-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Duan Y, Ma G, Huang X, D'Amore PA, Zhang F, Lei H
  TITLE     The CRISPR/Cas9-created MDM2 T309G enhances vitreous-induced 
            expression of MDM2 and proliferation and survival of cells.
  JOURNAL   J Biol Chem. 2016 May 31. pii: jbc.M116.729467.
  PUBMED    27246850
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 May 31. pii: jbc.M116.729467."
COMMENT     Sequence Label: pAAV-U6-sgRNA-CMV-GFP
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..238
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      280..289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(328..531)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(357..377)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        532..835
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
       61 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
      121 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
      181 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
      241 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtagcg
      301 ctagcggatc tgacggttca ctaaaccagc tctgcttata tagacctccc accgtacacg
      361 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      421 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
      481 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
      541 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
      601 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
      661 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
      721 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
      781 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
      841 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcga
      901 ccgggccccg gtaccctcta gaaaaaaagc accgactcgg tgccactttt tcaagttgat
      961 aacggactag ccttatttta acttgctatt tctagctc
//
