LOCUS       sequence-167536-       11201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA865-5p  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM865-5p
FEATURES             Location/Qualifiers
     source          1..11201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2397..2777
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2783..3334
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3344..3596
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3682..3706
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4131..4925
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5012..5600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5754..5771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5786..5926
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5852..5871)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6012..6034
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6270..6464
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6530..7603)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8032..8661)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9962..9986
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10174..10196
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10188..10205
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10189..10205
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10230..10937)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttctca
      361 attagatcta ccaaattcat gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatct caattagatc taccaaattc atgaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctgta ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagtcccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgatacg taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggtcaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaag aaaggcggac aggtatccgg taatctgctg cttgcaaacg cgtaatctgc
     2341 tgcttgcaaa cttaattaac tctctagact cacctaggat ccatgaattc tgagattttt
     2401 caaatcagtg cgcaagacgt gacgtaagta tccgagtcag tttttatttt tctactaatt
     2461 tggtcgttta tttcggcgtg taggacatgg caaccgggcc tgaatttcgc gggtattctg
     2521 tttctattcc aactttttct tgatccgcag ccattaacga cttttgaata gatacgctga
     2581 cacgccaagc ctcgctagtc aaaagtgtac caaacaacgc tttacagcaa gaacggaatg
     2641 cgcgtgacgc tcgcggtgac gccatttcgc cttttcagaa atggataaat agccttgctt
     2701 cctattatat cttcccaaat taccaataca ttacactagc atctgaattt cataaccaat
     2761 ctcgatacac caaatcgaat cgatgagccc agaacgacgc ccggccgaca tccgccgtgc
     2821 caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg agacaagcac
     2881 ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc tcgtccgtct
     2941 gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg gcatcgccta
     3001 cgcgggcccc tggaaggcac gcaacgccta cgactggacg gccgagtcga ccgtgtacgt
     3061 ctccccccgc caccagcgga cgggactggg ctccacgctc tacacccacc tgctgaagtc
     3121 cctggaggca cagggcttca agagcgtggt cgctgtcatc gggctgccca acgacccgag
     3181 cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc atgctgcggg cggccggctt
     3241 caagcacggg aactggcatg acgtgggttt ctggcagctg gacttcagcc tgccggtacc
     3301 gccccgtccg gtcctgcccg tcaccgagat ttgactgcag aatcttggac tcccatgttg
     3361 gcaaaggcaa ccaaacaaac aatgaatgat ccgctcctgc atatggggcg gtttgagtat
     3421 ttcaactgcc atttgggctg aattgaagac atgctcctgt cagaaattcc gtgatcttac
     3481 tcaatattca gtaatctcgg ccaatatcct aaatgtgcgt ggctttatct gtctttgtat
     3541 tgtttcatca attcatgtaa cgtttgcttt tcttatgaat tttcaaataa attatcagat
     3601 ccgagctcga attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     3661 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     3721 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     3781 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     3841 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     3901 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc
     3961 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     4021 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     4081 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     4141 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     4201 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     4261 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     4321 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     4381 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     4441 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     4501 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     4561 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     4621 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     4681 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     4741 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     4801 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     4861 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     4921 tttagtacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5161 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5221 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5281 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5341 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5401 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5461 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5521 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5581 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5641 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5701 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5761 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     5821 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5881 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     5941 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     6001 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     6061 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     6121 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     6181 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     6241 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     6301 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     6361 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     6421 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     6481 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     6541 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     6601 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     6661 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     6721 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     6781 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     6841 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     6901 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     6961 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     7021 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     7081 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     7141 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
     7201 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
     7261 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
     7321 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
     7381 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
     7441 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
     7501 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
     7561 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
     7621 gggggttcca gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc
     7681 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
     7741 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
     7801 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
     7861 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
     7921 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
     7981 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
     8041 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
     8101 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
     8161 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
     8221 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
     8281 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
     8341 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
     8401 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
     8461 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
     8521 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
     8581 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
     8641 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
     8701 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
     8761 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
     8821 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
     8881 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
     8941 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
     9001 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
     9061 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
     9121 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
     9181 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
     9241 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
     9301 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
     9361 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
     9421 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
     9481 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
     9541 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
     9601 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
     9661 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
     9721 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
     9781 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
     9841 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
     9901 ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta
     9961 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga
    10021 caatctgatc caagctcaag ctgctctagc attcgccatt caggctgcgc aactgttggg
    10081 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10141 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10201 ccagtgccaa gcttgcatgc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt
    10261 cgccctggac ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg
    10321 gggcccacat acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg
    10381 ccgccgtgct ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata
    10441 ctcaacttca aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga
    10501 gcgttattag ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat
    10561 ttgaataaga tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa
    10621 attggatttg taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc
    10681 gctataaacc tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt
    10741 agggtacaat cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt
    10801 gatatattca ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg
    10861 ttttttacaa cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc
    10921 atatctcatt aaagcaggac tctagggact agtcccgggt cttaattaag tttgcaagca
    10981 gcagattacg cgcagaaaaa aaggatctca aggagatcct ttggatcaca aaaaaaagcc
    11041 cgctcattag gcgggttaaa ttcccgggta ctgcagccga ttcattaatg caggttaacc
    11101 tggcttatcg aaattaatac gactcaccat agggagaccg gcagatctga tccctactcc
    11161 aaaaatgtca aagatacagt ctcagaagac caaagggcta t
//
