LOCUS       sequence-167539-       11518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA863-3p  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM863-3p
FEATURES             Location/Qualifiers
     source          1..11518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        125..470
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     413..433
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        complement(1368..1386)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1369..1386)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2025..2044
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2147..2152,2153..2169,2170..2175))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2437..3144
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3169..3186)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3169..3185)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3178..3200)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3388..3412
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4713..5342
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5771..6844
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6910..7104
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7340..7362)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7448..7588
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7503..7522
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7603..7620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7774..8362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8449..9243)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9668..9692
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9778..10030)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(10040..10591)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10597..10977)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11217..11236
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11217..11235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
       61 acgcacaatc ccacccctac tccaaaaatg tcaaagatac tgtctcagaa gaccaaaggg
      121 ctattgagac ttttcaacaa agggtaattt cgggaaacct cctcggattc cattgcccag
      181 ctatctgtca cctcaccgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
      241 attgcgataa aggaaaggct atcattcaag atgcctctgc cgacagtggt cccaaagatg
      301 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      361 aagtggattg atgtgacatc tccactgaca taagggatga cgcacaaccc cactatcctt
      421 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca gcccaagctt
      481 attatgtctt gcgattgctc tcaagttgtt gttgttatgg tctaatttaa atatggtcta
      541 aagaagaaga atattatgtc ttgcgattgc tctcaagaat tcggtacgct gaaatcacca
      601 gtctctctct acaaatctat ctctctctat tttctccata aataatgtgt gagtagtttc
      661 ccgataaggg aaattagggt tcttataggt ttcgctcatg tgttgagcat ataagaaacc
      721 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
      781 caaaatccag tactaaaatc cagatctcct aaagtcccta tagatctttg tcgtgaatat
      841 aaaccagacg cgagacgact agacctggag cccagacgcc gttcgaagct agaagtaccg
      901 cttaggcagg aggccgttag ggaaaagatg ctaaggcagg gttggttacg ttggctcccc
      961 cgtaggtttg gtttaaatat gatgaagtgg acggaaggaa ggaggaagac aaggaaggat
     1021 aaggttgcag gccctgtgca aggtaagaag atggaaattt gatagaggta cgctactata
     1081 cttatactat acgctaaggg aatgattgta tttataccct atacccccta ataacccctt
     1141 atcaatttaa gaaataatcc gcataagccc ccgcttaaaa attggtatca gagccatgaa
     1201 taggtctatg accaaaactc aagaggataa aacctcacca aaatacgaaa gagtccttaa
     1261 ctctaaagat aaaagatctt tcaagatcaa aactagttcc ctcacaccgg tgacggggat
     1321 cgcatgcgac ctctagacca tcacattgga gcttcagctg ctcgagttct atagtgtcac
     1381 ctaaatcgta tgcgtatgat acataaggtt atgtattaat tgtagccgcg ttcttacgtc
     1441 gacgtaccaa ttacgccccg ccctgtcact catcgcagta ctgttgtaat tcattaagca
     1501 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     1561 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1621 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     1681 aaaacatatt ctcaatgaac cctttaggga aataggccag gttttcaccg taacacgcca
     1741 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     1801 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1861 ttaccagctc accgtctttc attgccatac ggaactccgg atgagcattc atcaggcggg
     1921 caagagtgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1981 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2041 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtaattt
     2101 ctttctccat tttagcttcc ttagctcctg aaaatctcga taactcatta gagcttatcg
     2161 atgataagct gtcaaacatg agaatttagc ccgcctaatg agcgggcttt ttgttgtgat
     2221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttcccccct
     2341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2401 tttcttaatt aagacccggg actagtccct agagtcctgc tttaatgaga tatgcgagac
     2461 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
     2521 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
     2581 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
     2641 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
     2701 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
     2761 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
     2821 aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga
     2881 agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc
     2941 cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta
     3001 accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga
     3061 agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg
     3121 cgacaacatg tcgaggctca gcaggacctg caggcatgca agcttggcac tggccgtcgt
     3181 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3241 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3301 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
     3361 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     3421 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     3481 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     3541 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
     3601 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     3661 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     3721 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     3781 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     3841 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     3901 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     3961 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     4021 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     4081 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     4141 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     4201 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     4261 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     4321 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     4381 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     4441 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     4501 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     4561 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     4621 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     4681 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     4741 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     4801 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     4861 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     4921 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     4981 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     5041 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     5101 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     5161 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     5221 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     5281 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     5341 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     5401 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     5461 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     5521 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     5581 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     5641 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     5701 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
     5761 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     5821 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     5881 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     5941 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     6001 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     6061 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     6121 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     6181 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     6241 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     6301 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     6361 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     6421 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     6481 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     6541 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     6601 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     6661 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     6721 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     6781 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     6841 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     6901 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     6961 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     7021 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     7081 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
     7141 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     7201 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     7261 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
     7321 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7381 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7441 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     7501 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     7561 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     7621 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7681 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7741 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7801 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7861 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7921 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7981 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8041 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8101 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8161 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8221 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8281 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8341 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8401 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
     8461 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
     8521 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
     8581 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
     8641 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
     8701 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
     8761 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
     8821 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
     8881 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
     8941 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
     9001 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
     9061 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
     9121 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
     9181 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
     9241 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
     9301 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
     9361 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
     9421 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
     9481 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
     9541 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
     9601 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     9661 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     9721 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcgagct cggatctgat
     9781 aatttatttg aaaattcata agaaaagcaa acgttacatg aattgatgaa acaatacaaa
     9841 gacagataaa gccacgcaca tttaggatat tggccgagat tactgaatat tgagtaagat
     9901 cacggaattt ctgacaggag catgtcttca attcagccca aatggcagtt gaaatactca
     9961 aaccgcccca tatgcaggag cggatcattc attgtttgtt tggttgcctt tgccaacatg
    10021 ggagtccaag attctgcagt caaatctcgg tgacgggcag gaccggacgg ggcggtaccg
    10081 gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg
    10141 ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt
    10201 cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca
    10261 gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca
    10321 cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga
    10381 tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga
    10441 cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg
    10501 tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc
    10561 ggatgtcggc cgggcgtcgt tctgggctca tcgattcgat ttggtgtatc gagattggtt
    10621 atgaaattca gatgctagtg taatgtattg gtaatttggg aagatataat aggaagcaag
    10681 gctatttatc catttctgaa aaggcgaaat ggcgtcaccg cgagcgtcac gcgcattccg
    10741 ttcttgctgt aaagcgttgt ttggtacact tttgactagc gaggcttggc gtgtcagcgt
    10801 atctattcaa aagtcgttaa tggctgcgga tcaagaaaaa gttggaatag aaacagaata
    10861 cccgcgaaat tcaggcccgg ttgccatgtc ctacacgccg aaataaacga ccaaattagt
    10921 agaaaaataa aaactgactc ggatacttac gtcacgtctt gcgcactgat ttgaaaaatc
    10981 tcagaattca tggatcctag gtgagtctag agagttaatt aagtttgcaa gcagcagatt
    11041 accggatacc tgtccgcctt tcttaattaa gtttgcaagc agcagattac gcgcagaaaa
    11101 aaaggatctc aaggagatcc tttggatcac aaaaaaaaag cccgctcatt aggcgggtta
    11161 aattcccggg tactgcagcc gattcattaa tgcaggttaa cctggcttat cgaaattaat
    11221 acgactcact atagggagac cggcggatct gatccctact ccaaaaatgt caaagataca
    11281 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatttc gggaaacctc
    11341 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtgga aaaggaaggt
    11401 ggctcctaca aatgccatca ctgcgataaa ggaaaggcta tcattcaaga tgcctctgcc
    11461 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtgggaaa agaagacg
//
