LOCUS       sequence-167542-       11131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA861  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM861-3p
FEATURES             Location/Qualifiers
     source          1..11131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2327..2707
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2713..3264
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3274..3526
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3612..3636
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4061..4855
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4942..5530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5684..5701
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5716..5856
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5782..5801)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5942..5964
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6200..6394
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6460..7533)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7962..8591)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9892..9916
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10104..10126
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10118..10135
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10119..10135
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10160..10867)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11045..11064
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11045..11063
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaaggg cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttgtcc
      361 ttgaagactg catatccatc gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatgt ccttgaagac tgcatatcca tcgaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctggg
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaac tctctagact cacctaggat ccatgaattc tgagattttt caaatcagtg
     2341 cgcaagacgt gacgtaagta tccgagtcag tttttatttt tctactaatt tggtcgttta
     2401 tttcggcgtg taggacatgg caaccgggcc tgaatttcgc gggtattctg tttctattcc
     2461 aactttttct tgatccgcag ccattaacga cttttgaata gatacgctga cacgccaagc
     2521 ctcgctagtc aaaagtgtac caaacaacgc tttacagcaa gaacggaatg cgcgtgacgc
     2581 tcgcggtgac gccatttcgc cttttcagaa atggataaat agccttgctt cctattatat
     2641 cttcccaaat taccaataca ttacactagc atctgaattt cataaccaat ctcgatacac
     2701 caaatcgaat cgatgagccc agaacgacgc ccggccgaca tccgccgtgc caccgaggcg
     2761 gacatgccgg cggtctgcac catcgtcaac cactacatcg agacaagcac ggtcaacttc
     2821 cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc
     2881 tatccctggc tcgtcgccga ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc
     2941 tggaaggcac gcaacgccta cgactggacg gccgagtcga ccgtgtacgt ctccccccgc
     3001 caccagcgga cgggactggg ctccacgctc tacacccacc tgctgaagtc cctggaggca
     3061 cagggcttca agagcgtggt cgctgtcatc gggctgccca acgacccgag cgtgcgcatg
     3121 cacgaggcgc tcggatatgc cccccgcggc atgctgcggg cggccggctt caagcacggg
     3181 aactggcatg acgtgggttt ctggcagctg gacttcagcc tgccggtacc gccccgtccg
     3241 gtcctgcccg tcaccgagat ttgactgcag aatcttggac tcccatgttg gcaaaggcaa
     3301 ccaaacaaac aatgaatgat ccgctcctgc atatggggcg gtttgagtat ttcaactgcc
     3361 atttgggctg aattgaagac atgctcctgt cagaaattcc gtgatcttac tcaatattca
     3421 gtaatctcgg ccaatatcct aaatgtgcgt ggctttatct gtctttgtat tgtttcatca
     3481 attcatgtaa cgtttgcttt tcttatgaat tttcaaataa attatcagat ccgagctcga
     3541 attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     3601 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     3661 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     3721 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     3781 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     3841 tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg
     3901 atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa
     3961 ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg
     4021 gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc
     4081 accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc
     4141 tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga
     4201 cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct
     4261 ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa
     4321 tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag
     4381 ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat
     4441 atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg attacttact
     4501 gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga
     4561 tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc
     4621 ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat
     4681 tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc
     4741 gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat
     4801 caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct
     4861 agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     5101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5581 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5641 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5701 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5761 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     5821 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     5881 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5941 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
     6001 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     6061 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     6121 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     6181 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     6241 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     6301 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     6361 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
     6421 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     6481 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     6541 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     6601 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     6661 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     6721 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     6781 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     6841 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     6901 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     6961 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     7021 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
     7081 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
     7141 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
     7201 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
     7261 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
     7321 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
     7381 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     7441 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     7501 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
     7561 gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct
     7621 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     7681 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     7741 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     7801 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     7861 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     7921 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     7981 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
     8041 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
     8101 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
     8161 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
     8221 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
     8281 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
     8341 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
     8401 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
     8461 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
     8521 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
     8581 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
     8641 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
     8701 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
     8761 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
     8821 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
     8881 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
     8941 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
     9001 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
     9061 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
     9121 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
     9181 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
     9241 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
     9301 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
     9361 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
     9421 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
     9481 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
     9541 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
     9601 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
     9661 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
     9721 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
     9781 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
     9841 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
     9901 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
     9961 caagctcaag ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10021 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10081 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    10141 gcttgcatgc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
    10201 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
    10261 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
    10321 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
    10381 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
    10441 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
    10501 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
    10561 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
    10621 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
    10681 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
    10741 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
    10801 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
    10861 aaagcaggac tctagggact agtcccgggt cttaattaag tttgcaagca gcagattacg
    10921 cgcagaaaaa aaggatctca aggagatcct ttggatcaca aaaaaaagcc cgctcattag
    10981 gcgggttaaa ttcccgggta ctgcagccga ttcattaatg caggttaacc tggcttatcg
    11041 aaattaatac gactcactat agggagaccg gcagatctga tccctactcc aaaaatgtca
    11101 aagatacagt ctcagaagac caaagggcta t
//
