LOCUS       sequence-167544-         786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA860  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM860
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     119..138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(280..285,286..302,303..308)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(411..430)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttgaaatct cagaattcat ggatcctagg tgagtctaga gagttaatta agaaaggcgg
       61 acaggtatgg gtaagcggca gggtcggaac aggagagcgc acgagggagc tcccaggggg
      121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggatcaca
      241 acaaaaagcc cgctcattag gcgggctaaa ttctcatgtt tgacagctta tcatcgataa
      301 gctttaatga gttatcgaga ttttcaggaa ctaaggaagc taaaatggag aaagaaatca
      361 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      421 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      481 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      541 tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtaatatggg
      601 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      661 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
      721 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
      781 cagcca
//
