LOCUS       sequence-167548-        4854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BiFC vector for transient expression of frGC155-MXMT in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Fujii Y, Kodama Y
  TITLE     In planta comparative analysis of improved green fluorescent 
            proteins with reference to fluorescence intensity and bimolecular 
            fluorescence complementation ability.
  JOURNAL   Plant Biotech. 2015; 32(1): 81-87.
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotech."; date: "2015"; volume: " 32(1)"; pages: " 81-87"
COMMENT     Sequence Label: 35S-frGC155-MXMT-NosT
FEATURES             Location/Qualifiers
     source          1..4854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..365
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     308..328
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    385..440
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      1872..2124
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2162..2178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2197..2213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2210..2232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2221..2237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2245..2251,2252..2269,2270..2275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2290..2311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2428..2445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2599..3187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2679..2698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3358..4149,4150..4218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3981..4000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4219..4323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4391..4409
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4447..4469)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4569..4588
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4782..4804
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4796..4813
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4797..4813
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcagaagac cgagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
       61 ggattccatt gcccagctat ctgtcactta tcgaaaggac agtagaaaag gaaggtggct
      121 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      181 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      241 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      301 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      361 ggacaggctt cttgagatcc ttcaacaatt accaacaaca acaaacaaca aacaacatta
      421 caattactat ttacaattac agtcgactct agaggatcca tggccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagatg
      721 gagctccaag aagtcctgca tatgaatgaa ggtgaaggcg atacaagcta cgccaagaat
      781 gcatcctaca atctggctct tgccaaggtg aaacctttcc ttgaacaatg catacgagaa
      841 ttgttgcggg ccaacttgcc caacatcaac aagtgcatta aagttgcgga tttgggatgc
      901 gcttctggac caaacacact tttaacagtg cgggacattg tgcaaagtat tgacaaagtt
      961 ggccaggaag agaagaatga attagaacgt cccaccattc agatttttct gaatgatctt
     1021 ttccaaaatg atttcaattc ggttttcaag ttgctgccaa gcttctaccg caaactcgag
     1081 aaagaaaatg gacgcaagat aggatcgtgc ctaataagcg caatgcctgg ctctttctac
     1141 ggcagactct tccccgagga gtccatgcat tttttgcact cttgttacag tgttcattgg
     1201 ttatctcagg ttcccagcgg tttggtgatt gaattgggga ttggtgcaaa caaagggagt
     1261 atttactctt ccaaaggatg tcgtccgccc gtccagaagg catatttgga tcaatttacg
     1321 aaagatttta ccacatttct aaggattcat tcgaaagagt tgttttcacg tggccgaatg
     1381 ctccttacct gcatttgtaa agtagatgaa ttcgacgaac cgaatcccct agacttactt
     1441 gacatggcaa taaacgactt gattgttgag ggacttctgg aggaagaaaa attggatagt
     1501 ttcaatattc cattctttac accttcagca gaagaagtaa agtgcatagt tgaggaggaa
     1561 ggttcttgcg aaattttata tctggagact tttaaggccc attatgatgc tgccttctct
     1621 attgatgatg attacccagt aagatcccat gaacaaatta aagcagagta tgtggcatca
     1681 ttaattagat cagtttacga acccatcctc gcaagtcatt ttggagaagc tattatgcct
     1741 gacttattcc acaggcttgc gaagcatgca gcaaaggttc tccacatggg caaaggctgc
     1801 tataataatc ttatcatttc tctcgccaaa aagccagaga agtcagacgt gtaagcggcc
     1861 gcccggctgc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     1921 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     1981 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     2041 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     2101 cggtgtcatc tatgttacta gatccgatga taagctgtca aacatagaat tcgtaatcat
     2161 ggtcatagct gtttcctgtg tgaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     2221 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2281 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2461 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2761 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3001 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3241 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3301 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3361 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3421 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3481 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3541 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3601 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3661 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3721 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3781 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3841 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3901 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3961 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4021 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4081 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4141 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4201 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4261 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4321 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4381 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4441 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4501 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     4561 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     4621 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     4681 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     4741 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     4801 aacgacggcc agtgccaagc ttgcatgcct gcaggtcgat ctagaggatc cccc
//
