LOCUS       sequence-167550-        3685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP-based BiFC vector for transient expression of CC (155-238) in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBCC155
FEATURES             Location/Qualifiers
     source          1..3685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..367
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     310..330
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    387..442
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     656..677
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      741..993
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1031..1047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1031..1047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1044..1066)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1055..1071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1079..1085,1086..1103,1104..1109))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1124..1145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1262..1279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1433..2021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1513..1532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2192..2983,2984..3052))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2815..2834
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3053..3157)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3225..3243
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3281..3303)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3403..3422
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3616..3638
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3630..3647
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3631..3647
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
       61 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
      121 ctcctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      181 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      241 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      301 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      361 gaggacaggc ttcttgagat ccttcaacaa ttaccaacaa caacaaacaa caaacaacat
      421 tacaattact atttacaatt acagtcgact ctagaggatc catggccgac aagcagaaga
      481 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      541 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      601 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
      661 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
      721 aaagcggccg cccggctgca gatcgttcaa acatttggca ataaagtttc ttaagattga
      781 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
      841 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
      901 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
      961 tatcgcgcgc ggtgtcatct atgttactag atccgatgat aagctgtcaa acatgagaat
     1021 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1081 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1141 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1201 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1261 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1321 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1381 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1441 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1501 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1561 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1621 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1681 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1741 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1801 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1861 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1921 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1981 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2041 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2101 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2161 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2221 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2281 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2341 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2401 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2461 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2521 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2581 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2641 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2701 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2761 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2821 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2881 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2941 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3001 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3061 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3121 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3181 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3241 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3301 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3361 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     3421 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3481 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     3541 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     3601 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
     3661 gcctgcaggc tctagaggat ccccc
//
