LOCUS       sequence-167558-       11136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA854  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM854a-e
FEATURES             Location/Qualifiers
     source          1..11136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..46
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     polyA_signal    211..387
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(949..967)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(950..967)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1606..1625
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1728..1733,1734..1750,1751..1756))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2018..2725
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(2750..2767)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2750..2766)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2759..2781)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2969..2993
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4294..4923
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5352..6425
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6491..6685
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6921..6943)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7029..7169
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7084..7103
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7184..7201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7355..7943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8030..8824)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9249..9273
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9359..9611)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9621..10172)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10178..10558)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     10750..10769
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10750..10768
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     join(11128..11136,1..12)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagggaca gcccaagctt
       61 ctcctcctcc cctatatcct catcgttgtt gttgttatgg tctaatttaa atatggtcta
      121 aagaagaaga atctcctcct cccctatatc ctcatcgaat tcggtacgct gaaatcacca
      181 gtctctctct acaaatctat ctctctctat tttctccata aataatgtgt gagtagtttc
      241 ccgataaggg aaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
      301 ccttagtatg tatttgtatt tgtaaaatac ttctatccat aaaatttcta attcctaaaa
      361 ccaaaatcca gtactaaaat ccagatctcc taaagtccct atagatcttt gtcgtgaata
      421 taaaccagac acgagacgac tagacctgga gcccagacgc cgttcgaagc tagaagtacc
      481 gcttaggcag gaggccgtta gggaaaagat gctaaggcag ggttggttac gttgactccc
      541 ccgtaggttt ggtttaaata tgatgaagtg gacggaagga aggaggaaga caaggaagga
      601 taaggttgca ggccctgtgc aaggtaagaa gatggaaatt tgatagaggt acgctactat
      661 acttatacta tacgctaagg gaatgattgt atttataccc tataccccct aataacccct
      721 tatcaattta agaaataatc cgcataagcc cccgcttaaa aattggtatc agggccatga
      781 ataggtctat gaccaaaact caagaggata aaacctcacc aaaatacgaa agagtcctta
      841 actctaaaga taaaagatct ttcaagatca aaactagttc cctcacaccg gtgacgggga
      901 tcgcatgcga cctctagacc atcacattgg agcttcagct gctcgagttc tatagtgtca
      961 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttcttacgt
     1021 cgacgtacca attacgcccc gccctgtcac tcatcgcagt actgttgtaa ttcattaagc
     1081 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     1141 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     1201 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     1261 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     1321 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     1381 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     1441 attaccggct caccgtcttt cattgccata cggaactccg gatgagcatt catcaggcgg
     1501 gcaaggatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     1561 aaggccgtaa tatccagctg aacggtctgg ctataggtac attgagcaac tgactgaaat
     1621 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     1681 tctttctcca ttttagcttc cttagctcct gaaaatctcg ataactcatt aaagcttatc
     1741 gatgataagc tgtcaaacat gagaatttag cccgcctaat gagcgggctt tttgttgtga
     1801 tccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttcccccc
     1921 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1981 ctttcttaat taagacccgg gactagtccc tagagtcctg ctttaatgag atatgcgaga
     2041 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     2101 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     2161 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     2221 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     2281 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     2341 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     2401 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     2461 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     2521 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     2581 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     2641 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     2701 gcgacaacat gtcgaggctc agcaggacct gcaggcatgc aagcttggca ctggccgtcg
     2761 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2821 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2881 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt
     2941 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     3001 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     3061 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     3121 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     3181 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     3241 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     3301 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     3361 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     3421 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     3481 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     3541 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     3601 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     3661 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     3721 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     3781 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     3841 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     3901 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     3961 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     4021 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     4081 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     4141 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     4201 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     4261 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     4321 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     4381 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     4441 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     4501 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     4561 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     4621 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     4681 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     4741 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     4801 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     4861 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     4921 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     4981 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     5041 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     5101 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     5161 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     5221 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     5281 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
     5341 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     5401 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     5461 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     5521 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     5581 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     5641 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     5701 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     5761 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     5821 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     5881 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     5941 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     6001 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     6061 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     6121 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     6181 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     6241 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     6301 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     6361 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     6421 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     6481 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     6541 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     6601 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     6661 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     6721 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     6781 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     6841 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     6901 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     6961 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7021 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     7081 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7141 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     7201 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     7801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7981 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     8041 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     8101 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     8161 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     8221 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     8281 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     8341 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     8401 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     8461 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     8521 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     8581 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     8641 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     8701 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     8761 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     8821 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     8881 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
     8941 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     9001 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     9061 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
     9121 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
     9181 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
     9241 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
     9301 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgagc tcggatctga
     9361 taatttattt gaaaattcat aagaaaagca aacgttacat gaattgatga aacaatacaa
     9421 agacagataa agccacgcac atttaggata ttggccgaga ttactgaata ttgagtaaga
     9481 tcacggaatt tctgacagga gcatgtcttc aattcagccc aaatggcagt tgaaatactc
     9541 aaaccgcccc atatgcagga gcggatcatt cattgtttgt ttggttgcct ttgccaacat
     9601 gggagtccaa gattctgcag tcaaatctcg gtgacgggca ggaccggacg gggcggtacc
     9661 ggcaggctga agtccagctg ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg
     9721 gccgcccgca gcatgccgcg gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg
     9781 tcgttgggca gcccgatgac agcgaccacg ctcttgaagc cctgtgcctc cagggacttc
     9841 agcaggtggg tgtagagcgt ggagcccagt cccgtccgct ggtggcgggg ggagacgtac
     9901 acggtcgact cggccgtcca gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg
     9961 atgccggcga cctcgccgtc cacctcggcg acgagccagg gatagcgctc ccgcagacgg
    10021 acgaggtcgt ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt
    10081 gtctcgatgt agtggttgac gatggtgcag accgccggca tgtccgcctc ggtggcacgg
    10141 cggatgtcgg ccgggcgtcg ttctgggctc atcgattcga tttggtgtat cgagattggt
    10201 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
    10261 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
    10321 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
    10381 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
    10441 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
    10501 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
    10561 ctcagaattc atggatccta ggtgagtcta gagagttaat taagtttgca agcagcagat
    10621 tacgcgcaga aaaaaaggat ctcaaggaga tcctttggat cacaaaaaaa aagcccgctt
    10681 attaggcggg ttaaattccc gggtactgca gccgattcat taatgcaggt taacctggct
    10741 tatcgaaatt aatacgactc actataggga gaccggcaga tctgatccct actccaaaaa
    10801 tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
    10861 ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
    10921 ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca
    10981 agatgcctcc gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtggk
    11041 agaagaagac gtysytccaa ccacgtcttc aaagcaagtg gattgatgtg acatctccac
    11101 tgacataagg gatgacgcac aaccccacta tccttc
//
