LOCUS       sequence-16756-e        1326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4041
FEATURES             Location/Qualifiers
     source          1..1326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             286..306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             328..348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             370..390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             412..432
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(475..503)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cngnanttac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
       61 ggctgacctc nctcacaacg atggatacgc taacaacttg gaaatgaaat aagcttgcat
      121 gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc ctatcgattc gcggccgctg
      181 tacaccccaa aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt
      241 caggaggacc cttggagggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt
      301 aaggtaccgg cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggcagaaaaa
      361 atgactgctc caaagaagaa gcgtaaggta ccggcagaaa aaatgactgc tccaaagaag
      421 aagcgtaagg taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc
      481 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      541 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      601 ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
      661 tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca
      721 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      781 agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt
      841 actaactaac catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc
      901 cttgttaata gaatcgagtt aaaaggtatt gattttaaga agatggaaac attcttggac
      961 ncaattggaa tacaactata actcacccat gtatacttca tggcanacaa cnaaagaatg
     1021 gaatcaagtt gtagtttaac ntgatttnct aactaactat ctgattaant ttcnaactcc
     1081 aaataaccca cntgaaatgn agcntcacta cnnacctttc acaaatctcc attgggangg
     1141 cctgtcttta caaaanctta ctgnccnnat ntgccttnaa aancnanaaa nnaaccctgg
     1201 cctctggtng acccgcngga tacnnggggg anantaaaan cccncntnga ttcnnccccc
     1261 cnaatcggna acnccccctt attcantaan ggaaggnttc ccaaaaancc nggttttnnn
     1321 caaaaa
//
