LOCUS       sequence-167560-       11519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA847  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM847
FEATURES             Location/Qualifiers
     source          1..11519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2375..2755
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2761..3312
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3322..3574
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3660..3684
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4109..4903
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4990..5578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5732..5749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5764..5904
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5830..5849)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5990..6012
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6248..6442
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6508..7581)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8010..8639)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9940..9964
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10152..10174
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10166..10183
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10167..10183
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10208..10915)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaag ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttcatc
      361 aagaagactg agaggagtga gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatca tcaagaagac tgagaggagt gagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taggggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactgga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaagatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcattcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaggg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggcttcttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcggcgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaag aaaggcggac aggtatccgg taatctgctg cttgcaaact taattaactc
     2341 tctagactca cctaggatcc atgaattctg agatttttca aatcagtgcg caagacgtga
     2401 cgtaagtatc cgagtcagtt tttatttttc tactaatttg gtcgtttatt tcggcgtgta
     2461 ggacatggca accgggcctg aatttcgcgg gtattctgtt tctattccaa ctttttcttg
     2521 atccgcagcc attaacgact tttgaataga tacgctgaca cgccaagcct cgctagtcaa
     2581 aagtgtacca aacaacgctt tacagcaaga acggaatgcg cgtgacgctc gcggtgacgc
     2641 catttcgcct tttcagaaat ggataaatag ccttgcttcc tattatatct tcccaaatta
     2701 ccaatacatt acactagcat ctgaatttca taaccaatct cgatacacca aatcgaatcg
     2761 atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg
     2821 gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg
     2881 caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc
     2941 gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggcccctg gaaggcacgc
     3001 aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg
     3061 ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag
     3121 agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc
     3181 ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac
     3241 gtgggtttct ggcagctgga cttcagcctg ccggtaccgc cccgtccggt cctgcccgtc
     3301 accgagattt gactgcagaa tcttggactc ccatgttggc aaaggcaacc aaacaaacaa
     3361 tgaatgatcc gctcctgcat atggggcggt ttgagtattt caactgccat ttgggctgaa
     3421 ttgaagacat gctcctgtca gaaattccgt gatcttactc aatattcagt aatctcggcc
     3481 aatatcctaa atgtgcgtgg ctttatctgt ctttgtattg tttcatcaat tcatgtaacg
     3541 tttgcttttc ttatgaattt tcaaataaat tatcagatcc gagctcgaat taattcggcg
     3601 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
     3661 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
     3721 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
     3781 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
     3841 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
     3901 agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat
     3961 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
     4021 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
     4081 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
     4141 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
     4201 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
     4261 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
     4321 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
     4381 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
     4441 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
     4501 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
     4561 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
     4621 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
     4681 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     4741 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     4801 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     4861 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag aatgcatgac
     4921 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4981 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5041 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5101 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5161 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5221 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5281 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5341 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5401 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5461 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5521 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5581 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5641 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5701 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5761 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     5821 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     5881 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     5941 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6001 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
     6061 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     6121 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     6181 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     6241 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     6301 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     6361 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     6421 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
     6481 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     6541 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     6601 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     6661 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     6721 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     6781 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     6841 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     6901 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     6961 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     7021 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     7081 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     7141 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     7201 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     7261 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     7321 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     7381 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     7441 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     7501 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     7561 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     7621 ggccggcagg caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     7681 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     7741 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     7801 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     7861 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     7921 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     7981 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
     8041 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
     8101 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
     8161 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
     8221 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
     8281 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
     8341 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
     8401 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
     8461 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
     8521 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
     8581 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
     8641 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
     8701 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
     8761 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
     8821 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
     8881 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
     8941 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
     9001 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
     9061 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
     9121 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
     9181 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
     9241 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
     9301 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
     9361 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
     9421 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
     9481 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
     9541 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
     9601 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
     9661 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
     9721 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
     9781 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga
     9841 accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt
     9901 aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct
     9961 gtcaaacact gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct
    10021 gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10081 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10141 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct
    10201 gcaggtcctg ctgagcctcg acatgttgtc gcaaaattcg ccctggaccc gcccaacgat
    10261 ttgtcgtcac tgtcaaggtt tgacctgcac ttcatttggg gcccacatac accaaaaaaa
    10321 tgctgcataa ttctcggggc agcaagtcgg ttacccggcc gccgtgctgg accgggttga
    10381 atggtgcccg taactttcgg tagagcggac ggccaatact caacttcaag gaatctcacc
    10441 catgcgcgcc ggcggggaac cggagttccc ttcagtgagc gttattagtt cgccgctcgg
    10501 tgtgtcgtag atactagccc ctggggcact tttgaaattt gaataagatt tatgtaatca
    10561 gtcttttagg tttgaccggt tctgccgctt tttttaaaat tggatttgta ataataaaac
    10621 gcaattgttt gttattgtgg cgctctatca tagatgtcgc tataaaccta ttcagcacaa
    10681 tatattgttt tcattttaat attgtacata taagtagtag ggtacaatca gtaaattgaa
    10741 cggagaatat tattcataaa aatacgatag taacgggtga tatattcatt agaatgaacc
    10801 gaaaccggcg gtaaggatct gagctacaca tgctcaggtt ttttacaacg tgcacaacag
    10861 aattgaaagc aaatatcatg cgatcatagg cgtctcgcat atctcattaa agcaggactc
    10921 tagggactag tcccgggtct taattaagtt tgcaagcagc agattacgcg cagaaaaaaa
    10981 ggatctcaag gagatccttt ggatcacaaa aaaaaagtcc gctcattagg cgggttaaat
    11041 tcccgggtac tgcagccgat tcattaatgc aggttaacct ggcttatcga aattaatatg
    11101 actcactata gggagaccgg cagatctgat ccctactcca aaaacgtcaa agatacagtc
    11161 tcagaagacc aaagggctat tgagactttt caacaaaggg taatttcggg aaacctcctc
    11221 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc
    11281 tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatgc ctctgccgac
    11341 agtggtccca aagatggacc cccacccacg aggagcatcg tgggaaaaga agacgttcca
    11401 accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca
    11461 caatcccacc cctactccaa aaatgtcaaa gatactgtct cagaagacca aagggctat
//
