LOCUS       sequence-167563-       11726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA846  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM846
FEATURES             Location/Qualifiers
     source          1..11726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        complement(1501..1519)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1502..1519)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2158..2177
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2280..2285,2286..2302,2303..2308))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2569..3276
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3301..3318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3301..3317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3310..3332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3520..3544
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4845..5474
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5903..6976
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7042..7236
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7472..7494)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7580..7720
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7635..7654
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7735..7752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7906..8494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8581..9375)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9800..9824
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9910..10162)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(10172..10723)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10729..11109)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11301..11320
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11301..11319
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca actacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttaatt
      361 caagcaccta ttcaattcaa gttgttgttg ttatggtcta atttgaataa ttcaagcacc
      421 tattcaattc aagaattcgg tacgctgaaa tcaccaggcc atttggagag gacagcccaa
      481 gcttaattca agcacctatt caattcgcca tttaaatatg gtctaaagaa gaagaataat
      541 tcaagcacct attcaattca agaattcggt acgctgaaat caccagccat ttggagagga
      601 cagcccaagc ttaattcaag cacctattca attcaagttg ttgttgttat ggtctaattt
      661 aaatatggtc taaagaagaa gaataattca agcacctatt caattcaaga attcggtacg
      721 ctgaaatcac cagtctctct ctacaaatct atctctctct attttctcca taaataatgt
      781 gtgagtagtt tcccgataag ggaaattagg gttcttatag ggcttcgctc atgtgttgag
      841 catataagaa acccttagta tgtatttgta tttgtacaat acttctatca ataaaatttc
      901 taattcctaa aaccaaaatc cagtactaaa atccagatct cctaaagtcc ctatagatct
      961 ttgtcgtgaa tataaaccag acacgagacg actagacctg gagcccagac gccgttcgaa
     1021 gctagaagta ccgcttaggc aggaggccgt tagggaaaag atgctaaggc agggttggtt
     1081 acgttgactc ccccgtaggt ttggtttaaa tatgatgaag tggacggaag gaaggaggaa
     1141 gacaaggaag gataaggttg caggccctgt gcaaggtaag aagatggaaa tttgatagag
     1201 gtacgctact atacttatac tatacgctaa gggaatgatt gtatttatac cctatacccc
     1261 ctaataaccc cttatcaatt taagaaataa tccgcataag cccccgctta aaaattggta
     1321 tcagagccat gaataggtct atgaccaaaa ctcaagagga taaaacctca ccaaaataca
     1381 aaagagtcct taactctaaa gataaaagat ctttcaagat caaaactagt tccctcacac
     1441 cggtgacggg ggtcgcatgc gacctctaga ccatcacatt ggagcttcag ccgctcgagt
     1501 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     1561 gcgttcttac gtcgacgtac caattacgcc ccgccctgtc actcatcgca gtactgttgt
     1621 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     1681 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     1741 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     1801 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     1861 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     1921 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     1981 acactatccc atattaccag ctcaccgtct ttcattgcca tacggaaccc cggatgagca
     2041 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2101 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2161 actgactgaa atgcctcaaa atgttcttta cgataccatt gggatatatc aacggtggta
     2221 tatccagtga tttctttctc cattttagct tccttagctc ctgaaaatct cgataactca
     2281 ttaaagctta tcgatgataa gctgtcaaac atgagaattc agcccgccta atgagcgggc
     2341 tttttgttgt gatccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2461 gcgtttcccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2521 atacctgtcc gctttcttaa ttaagacccg ggactagtcc ctagagtcct gctttaatga
     2581 gatatgcgag acgcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta
     2641 aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat
     2701 atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt
     2761 accctactac ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt
     2821 atagcgacat ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat
     2881 ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta
     2941 ttcaaatttc aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat
     3001 aacgctcact gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag
     3061 ttgagtattg gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac
     3121 ggcggccggg taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg
     3181 ggccccaaat gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca
     3241 gggcgaattt tgcgacaaca tgtcgaggct cagcaggacc tgcaggcatg caagcttggc
     3301 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3361 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3421 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
     3481 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     3541 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     3601 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     3661 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     3721 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     3781 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     3841 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     3901 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     3961 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     4021 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     4081 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     4141 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     4201 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     4261 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     4321 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     4381 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     4441 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     4501 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     4561 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     4621 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     4681 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     4741 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     4801 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     4861 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     4921 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     4981 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     5041 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     5101 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     5161 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     5221 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     5281 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     5341 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     5401 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     5461 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     5521 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     5581 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     5641 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     5701 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     5761 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     5821 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     5881 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     5941 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     6001 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     6061 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     6121 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     6181 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     6241 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     6301 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     6361 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     6421 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     6481 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     6541 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     6601 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     6661 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     6721 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     6781 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     6841 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     6901 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     6961 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     7021 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     7081 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     7141 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     7201 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     7261 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     7321 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     7381 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     7441 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     7501 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7561 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     7621 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7681 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     7741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
     8581 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     8641 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     8701 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     8761 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     8821 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     8881 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     8941 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     9001 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     9061 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     9121 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     9181 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     9241 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     9301 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     9361 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     9421 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     9481 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     9541 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     9601 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     9661 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     9721 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     9781 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     9841 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcgag
     9901 ctcggatctg ataatttatt tgaaaattca taagaaaagc aaacgttaca tgaattgatg
     9961 aaacaataca aagacagata aagccacgca catttaggat attggccgag attactgaat
    10021 attgagtaag atcacggaat ttctgacagg agcatgtctt caattcagcc caaatggcag
    10081 ttgaaatact caaaccgccc catatgcagg agcggatcat tcattgtttg tttggttgcc
    10141 tttgccaaca tgggagtcca agattctgca gtcaaatctc ggtgacgggc aggaccggac
    10201 ggggcggtac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
    10261 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
    10321 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
    10381 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
    10441 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc
    10501 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
    10561 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
    10621 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
    10681 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catcgattcg atttggtgta
    10741 tcgagattgg ttatgaaatt cagatgctag tgtaatgtat tggtaatttg ggaagatata
    10801 ataggaagca aggctattta tccatttctg aaaaggcgaa atggcgtcac cgcgagcgtc
    10861 acgcgcattc cgttcttgct gtaaagcgtt gtttggtaca cttttgacta gcgaggcttg
    10921 gcgtgtcagc gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa aagttggaat
    10981 agaaacagaa tacccgcgaa attcaggccc ggttgccatg tcctacacgc cgaaataaac
    11041 gaccaaatta gtagaaaaat aaaaactgac tcggatactt acgtcacgtc ttgcgcactg
    11101 atttgaaaaa tctcagaatt catggatcct aggtgagtct agagagttaa ttaagtttgc
    11161 aagcagcaga ttacgcgcag aaaaaaagga tctcaaggag atcctttgga tcacaaaaaa
    11221 aaagcccgct cattaggcgg gttaaattcc cgggtactgc agccgattca ttaatgcagg
    11281 ttaacctggc ttatcgaaat taatacgact cactataggg agaccggcag atctgatccc
    11341 tactccaaaa atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
    11401 caaagggtaa tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
    11461 gaaaggacag tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
    11521 gctatcattc aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    11581 agcatcgtgg gaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac
    11641 atctccactg acgtaaggga tgacgcacaa tcccacccct actccaaaaa tgtcaaagat
    11701 actgtcttag aagaccaaag ggctat
//
