LOCUS       sequence-167564-       11133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA822  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM822
FEATURES             Location/Qualifiers
     source          1..11133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    508..684
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1246..1264)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1247..1264)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1903..1922
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2025..2030,2031..2047,2048..2053))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2315..3022
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3047..3064)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3047..3063)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3056..3078)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3266..3290
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4591..5220
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5649..6722
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6788..6982
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7218..7240)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7326..7466
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7381..7400
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7481..7498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7652..8240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8327..9121)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9546..9570
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9656..9908)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9918..10469)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10475..10855)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11047..11066
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11047..11065
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccgcccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttaaca
      361 tgtgcaaact atgcttcccg cgttgttgtt gttatggtct aatttaaata tggtctaaag
      421 aagaagaatc atgtgcaaat ctagcttccc gcagaattcg gtacgctgaa atcaccagtc
      481 tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag tagtttcccg
      541 ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
      601 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
      661 aaatccagta ctaaaatcca gatctcctaa agtccctata gatctttgtc gtgaatataa
      721 accagacacg agacgactag acctggagcc cagacgccgt tcgaagctag aagtaccgct
      781 taggcaggag gccgttaggg aaaagatgct aaggcagggt tggttacgtt gactcccccg
      841 taggtttggt ttaaatatga tgaagtggac ggaaggaagg aggaagacaa ggaaggataa
      901 ggttgcaggc cctgtgcaag gtaagaagat ggaaatttga tagaggtacg ctactatact
      961 tatactatac gctaagggaa tgattgtatt tataccctat accccctaat aaccccttat
     1021 caatttaaga aataatccgc ataagccccc gcttaaaaat tggtatcaga gccatgaata
     1081 ggtctatgac caaaactcaa gaggataaaa cctcaccaaa atacgaaaga gtccttaact
     1141 ctaaagataa aagatctttc aagatcaaaa ctagttccct cacaccggtg acggggatcg
     1201 catgcgacct ctagaccatc acattggagc ttcagctgct cgagttctat agtgtcacct
     1261 aaatcgtatg tgtatgatac ataatgttat gtattaattg tagccgcgtt cttacgtcga
     1321 cgtaccagtt acgccccgcc ctgtcactca tcgcagtact gttgtaattc attaagcatt
     1381 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1441 actttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1501 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     1561 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1621 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1681 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatt
     1741 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca
     1801 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1861 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1921 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgatttct
     1981 ttctccattt tagcttcctt agctcctgaa aatctcgata actcattaaa gcttatcgat
     2041 gataagctgt caaacatgag aatttagccc gcctaatgag cgggcttttt gttgtgatcc
     2101 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2161 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcccccctgg
     2221 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2281 tcttaattaa gacccgggac tagtccctag agtcctgctt taatgagata tgcgagacgc
     2341 ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat
     2401 gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac
     2461 tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc tactacttat
     2521 atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag cgacatctat
     2581 gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa ttttaaaaaa
     2641 agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca aatttcaaaa
     2701 gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg ctcactgaag
     2761 ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga gtattggccg
     2821 tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg gccgggtaac
     2881 cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc ccaaatgaag
     2941 tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc gaattttgcg
     3001 acaacatgtc gaggctcagc aggacctgca ggcatgcaag cttggcactg gccgtcgttt
     3061 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3121 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3181 tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc
     3241 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     3301 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     3361 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     3421 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
     3481 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     3541 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     3601 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     3661 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     3721 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     3781 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     3841 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     3901 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     3961 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     4021 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     4081 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     4141 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     4201 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     4261 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     4321 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     4381 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     4441 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     4501 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     4561 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     4621 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     4681 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     4741 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     4801 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     4861 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     4921 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     4981 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     5041 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     5101 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     5161 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     5221 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     5281 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     5341 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     5401 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     5461 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     5521 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     5581 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     5641 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     5701 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     5761 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     5821 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     5881 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     5941 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     6001 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     6061 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     6121 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     6181 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     6241 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     6301 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     6361 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     6421 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     6481 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     6541 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     6601 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     6661 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     6721 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc
     6781 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
     6841 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
     6901 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
     6961 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
     7021 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
     7081 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
     7141 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
     7201 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     7261 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     7321 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     7381 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     7441 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     7501 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7561 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7621 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7681 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7741 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7801 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7861 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7921 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7981 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8041 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8101 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8161 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8221 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8281 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat
     8341 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     8401 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     8461 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     8521 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     8581 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
     8641 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     8701 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     8761 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     8821 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     8881 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     8941 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     9001 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     9061 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
     9121 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
     9181 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
     9241 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
     9301 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
     9361 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
     9421 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
     9481 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
     9541 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
     9601 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgagctcg gatctgataa
     9661 tttatttgaa aattcataag aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga
     9721 cagataaagc cacgcacatt taggatattg gccgagatta ctgaatattg agtaagatca
     9781 cggaatttct gacaggagca tgtcttcaat tcagcccaaa tggcagttga aatactcaaa
     9841 ccgccccata tgcaggagcg gatcattcat tgtttgtttg gttgcctttg ccaacatggg
     9901 agtccaagat tctgcagtca aatctcggtg acgggcagga ccggacgggg cggtaccggc
     9961 aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc
    10021 gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg
    10081 ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc
    10141 aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg
    10201 gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg
    10261 ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg
    10321 aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc
    10381 tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg
    10441 atgtcggccg ggcgtcgttc tgggctcatc gattcgattt ggtgtatcga gattggttat
    10501 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc
    10561 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt
    10621 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat
    10681 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc
    10741 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag
    10801 aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt gaaaaatctc
    10861 agaattcatg gatcctaggt gagtctagag agttaattaa gtttgcaagc agcagattac
    10921 gcgcagaaaa aaaggatctc aaggagatcc tttggatcac aaaaaaaaaa cccgctcatt
    10981 aggcgggtta aattcccggg tactgcagcc gattcattaa tgcaggttaa cctggcttat
    11041 cgaaattaat acgactcact atagggagac cggcagatct gatccctact ccaaaaatgt
    11101 caaagataca gtctcagaag accaaagggc tat
//
