LOCUS       sequence-16757-e        1377 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4042
FEATURES             Location/Qualifiers
     source          1..1377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             671..691
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             713..733
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             755..775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             797..817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(860..888)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tactgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgacc
       61 tcactcacaa cgatggatac gctaacaact tggaaatgaa ataagcttgc atgcctgcag
      121 gtcgactcta gaggatcacc aaaaacggaa cgttgagctg gacggaaata gtggtaaagt
      181 gacatgatta tagtttgaag atttctaatt tcacaattag agcaaatgtt gttcggtatt
      241 tattttcaac ggtatttata ctattttcca cctttttcta gaacattcga gctgcttgtt
      301 gcaaaaggag ggcgactcac attcggtaca tggaaaagta gtgtacacaa taaagagacc
      361 cagatacatt ttccgtctgc gtctctttgc acccaccggg agtattttca aacgaatgca
      421 tctaggacct tctagaacat tctgtaaggc tgcagaatgc gggtatataa ggaaagcggg
      481 ctcagaggaa gccaacacgc tttgttctag tgcatctaaa aaacttcgaa aatcctcatc
      541 ggatcctatc gattcgcggc cgctgtacac cccaaaggac ccaaaggtat gtttcgaatg
      601 atactaacat aacatagaac attttcagga ggacccttgg agggtaccga gctcagaaaa
      661 aatgactgct ccaaagaaga agcgtaaggt accggcagaa aaaatgactg ctccaaagaa
      721 gaagcgtaag gtaccggcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggc
      781 agaaaaaatg actgctccaa agaagaagcg taaggtaccg gtagaaaaaa tgagtaaagg
      841 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      901 gcacaaattt tctgtcagtg gagagggtga aggggatgcc acatacggna aaacttaccc
      961 ttaaatttat tggccctact ggaaaactac ctgntccntg ggtaagttta aacatatata
     1021 tactanctaa cccntgatta tttaantttc ngcccacnct tgnccctact tnnngttagg
     1081 gggtccanng cttcccngaa aaccccnatc aatgaaacgg gctgactttt tcaaaaggnc
     1141 ctgcccnaag gtttgtnnng gaaaaanctn ttttttcaaa aggacgggaa cccnannacc
     1201 nttagttnaa ccggtcggna caaanaaccn aacattnaan tttcggggng aaanccagnt
     1261 ngnggggnac ccctnnttta aaaccnntna agggtttttt taaaaaaggn aaacttnntg
     1321 gccnnatggg aaaaccattt ccccccnggt ttcttgggaa aanaaaaagg anatntg
//
