LOCUS       sequence-167578-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the nucleus (can be used for colocalization
            studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pmScarlet-i_H2A_C1
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..735
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(173..191)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     441..460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaaccgtc agaatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggca
       61 gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      181 aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg
      241 tacggctcca gggccttcat caagcacccc gccgacatcc ccgactacta taagcagtcc
      301 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc
      361 gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc
      421 accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc
      481 accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat ggccctgcgc
      541 ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc caagaagccc
      601 gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc ccacaacgag
      661 gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagt
//
