LOCUS       sequence-16758-e        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4046
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             250..270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             292..312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             334..354
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             376..396
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(439..467)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtttactgat cttactaact aactattctc atttaaattt tcagagctta aaaatggctg
       61 aaatcactca caacgatgga tacgctaaca acttggaaat gaaataagct tgcatgcctg
      121 caggtcgact ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
      181 atactaacat aacatagaac attttcagga ggacccttga gggtaccgag ctcagaaaaa
      241 atgactgctc caaagaagaa gcgtaaggta ccggcagaaa aaatgactgc tccaaagaag
      301 aagcgtaagg taccggcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggca
      361 gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tagaaaaaat gagtaaagga
      421 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      481 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      541 aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa catatatata
      601 ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt
      661 gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc
      721 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      781 acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct
      841 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      901 aaagaagatg gaaacattct tggacacaat tggaatacaa ctataactca ccnatgtata
      961 catcntggca gacaaccaaa gaatggaatc cangtngtag tttaacntga tttnctaact
     1021 actatctgat ttaattttcg actccaatta acccacntga gatggagcgt cactgcnacn
     1081 tntcacaaat actcnntggg angccngtct ttcnnanacn tncngtcccc atngccnttc
     1141 aaaatccaca aaaaaaccct ggcctctgat ngaccccgcg gntncctgnn ggcaataaaa
     1201 acccncctng atnctncncc cnnanaccga aacanccccc ccgantnatt aangnaanga
     1261 tttctttcna nacttggctt gggttnggnn aaaaaaaaan aaattttttc cccccccccc
     1321 cccga
//
