LOCUS       sequence-16759-e        1359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4047
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             259..279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             301..321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             343..363
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             385..405
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(448..476)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cnngngttac atgatcttct aactaactat tctcatttaa attttcagag cttaaaaatg
       61 gctganctcn ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg
      121 cctgcaggtc gactctagag gatccccggg attggccaaa ggacccaaag gtatgtttcg
      181 aatgatacta acataacata gaacattttc aggaggaccc ttgcttggag ggtaccgagc
      241 tcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac cggcagaaaa aatgactgct
      301 ccaaagaaga agcgtaaggt accggcagaa aaaatgactg ctccaaagaa gaagcgtaag
      361 gtaccggcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt agaaaaaatg
      421 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      481 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      541 cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac
      601 atatatatac taactaaccc tgattattta aattttcagc caacacttgt cactactttc
      661 tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc
      721 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      781 aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
      841 tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaagg
      901 tattgatttt aaagaagatg gaaacattct tggacncaaa ttggaataca nctataactc
      961 acncnatgta tacctcatgg cagacaaaca aagaatggaa tccaagttgt angtttaacn
     1021 tgattttact anctaactaa tctgatttaa ttttcngaac ttcnaaatta gacccacntt
     1081 gaagatggaa gcgttcnctt accnaacctt ttccacaaat nctccattgg cgatggcctg
     1141 tcctttacnn aanaccntnc ctgtcccccn atntgccntt ncaaaattcc nacgaaaana
     1201 aaanccccng ncccttttgn ttngtaccnc ctgcnggatt ancctgggnn ggacaaattn
     1261 acaaancccc naacnttngn antncncnnc cncccccana nntcnggaaa nccccccccc
     1321 cccntgantt ncantttaaa ccgaanttnt tttttcctt
//
