LOCUS       sequence-167596-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad4 P130S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Moren A, Itoh S, Moustakas A, Dijke P, Heldin CH
  TITLE     Functional consequences of tumorigenic missense mutations in the 
            amino-terminal domain of Smad4.
  JOURNAL   Oncogene. 2000 Sep 7;19(38):4396-404.
  PUBMED    10980615
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2000-09-7"; volume: "19(38)"; pages: "4396-404"
COMMENT     Sequence Label: pcDNA3-Flag-Smad4 P130S
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             108..131
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac cgccatggac tacaaggacg
      121 atgatgacaa gggcgaattc gagaatatgt ctattacgaa tacaccaaca agtaatgatg
      181 cctgtctgag cattgtgcat agtttgatgt gccatagaca aggtggagag agtgaaacat
      241 ttgcaaaaag agcaattgaa agtttggtaa agaagctgaa ggagaaaaaa gatgaattgg
      301 attctttaat aacagctata actacaaatg gagctcatcc tagtaaatgt gttaccatac
      361 agagaacatt ggatgggagg cttcaggtgg ctggtcggaa aggatttcct catgtgatct
      421 atgcccgtct ctggaggtgg cctgatcttc acaaaaatga actaaaacat gttaaatatt
      481 gtcagtatgc gtttgactta aaatgtgata gtgtctgtgt gaattcatat cactacgaac
      541 gagttgtatc acctggaatt gatctctcag gattaacact gcagagtaat gctccatcaa
      601 gtatgatggt gaaggatgaa tatgtgcatg actttgaggg acagccatcg ttgtccactg
      661 aaggacattc aattcaaacc atccagcatc caccaagtaa tcgtgcatcg acagagacat
      721 acagcacccc agctctgtta gccccatctg agtctaatgc taccagcact gccaactttc
      781 ccaacattcc tgtggcttcc acaagtcagc ctgccagtat actggggggc agccatagtg
      841 aaggactgtt gcagatagca tcagggcctc agccaggaca gcagcagaat ggatttactg
//
