LOCUS       sequence-167598-       11469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA832-5p  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM832-5p
FEATURES             Location/Qualifiers
     source          1..11469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     1900..1919
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2022..2027,2028..2044,2045..2050))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2325..2705
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2711..3262
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3272..3524
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3610..3634
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4059..4853
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4940..5528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5682..5699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5714..5854
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5780..5799)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5940..5962
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6198..6392
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6458..7531)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7960..8589)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9890..9914
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10102..10124
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10116..10133
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10117..10133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10158..10865)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11044..11063
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11044..11062
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaggatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tgggaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcact tcatttggag aggacagccc aagctttttc
      361 gattccccta gatcccagca gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaattt tcgattcccc tagatcccag cagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agcttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca tagcccccgc ttaaaaattg gtatcagagc catgaatagg
     1081 tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt ccttaactct
     1141 aaagataaaa gatcttccaa gatcaaaact agttccctca caccggtgac ggggatcgca
     1201 tgcgacctct agaccatcac attggagctt cagctgctcg agttctatag tgtcacctaa
     1261 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttctt acgtcgacgt
     1321 accaattacg ccccgccctg tcgctcatcg cagtactgtt gtaattcatt aagcattctg
     1381 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1441 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1501 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1561 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1621 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1681 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatattacc
     1741 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
     1801 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1861 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1921 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttctttc
     1981 tccattttag cttccttagc tcctgaaaat ctcgataact cattaaagct tatcgatgat
     2041 aagctgtcaa acatgagaat ttagcccgcc taatgagcgg gctttttgtt gtgatccgcg
     2101 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2161 ggtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cccctggaag
     2221 ctccctcgcg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2281 taattaactc tctagactca cctaggatcc atgaattctg agatttttca aatcagtgcg
     2341 caagacgtga cgtaagtatc cgagtcagtt tttatttttc tactaatttg gtcgtttatt
     2401 tcggcgtgta ggacatggca accgggcctg aatttcgcgg gtattctgtt tctattccaa
     2461 ctttttcttg atccgcagcc attaacgact tttgaataga tacgctgaca cgccaagcct
     2521 cgctagtcaa aagtgtacca aacaacgctt tacagcaaga acggaatgcg cgtgacgctc
     2581 gcggtgacgc catttcgcct tttcagaaat ggataaatag ccttgcttcc tattatatct
     2641 tcccaaatta ccaatacatt acactagcat ctgaatttca taaccaatct cgatacacca
     2701 aatcgaatcg atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga
     2761 catgccggcg gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg
     2821 taccgagccg caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta
     2881 tccctggctc gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggcccctg
     2941 gaaggcacgc aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca
     3001 ccagcggacg ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca
     3061 gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca
     3121 cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa
     3181 ctggcatgac gtgggtttct ggcagctgga cttcagcctg ccggtaccgc cccgtccggt
     3241 cctgcccgtc accgagattt gactgcagaa tcttggactc ccatgttggc aaaggcaacc
     3301 aaacaaacaa tgaatgatcc gctcctgcat atggggcggt ttgagtattt caactgccat
     3361 ttgggctgaa ttgaagacat gctcctgtca gaaattccgt gatcttactc aatattcagt
     3421 aatctcggcc aatatcctaa atgtgcgtgg ctttatctgt ctttgtattg tttcatcaat
     3481 tcatgtaacg tttgcttttc ttatgaattt tcaaataaat tatcagatcc gagctcgaat
     3541 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     3601 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     3661 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     3721 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     3781 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     3841 taacgatgac agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat
     3901 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
     3961 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
     4021 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
     4081 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
     4141 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     4201 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     4261 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     4321 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     4381 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     4441 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
     4501 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
     4561 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     4621 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     4681 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     4741 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     4801 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
     4861 aatgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5701 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5761 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     5821 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     5881 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5941 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     6001 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     6061 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     6121 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     6181 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     6241 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     6301 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     6361 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
     6421 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     6481 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     6541 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     6601 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     6661 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     6721 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     6781 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     6841 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     6901 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     6961 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     7021 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     7081 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     7141 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     7201 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     7261 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
     7321 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
     7381 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
     7441 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
     7501 gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt
     7561 gccattgcag ggccggcagg caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
     7621 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
     7681 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
     7741 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
     7801 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
     7861 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
     7921 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
     7981 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
     8041 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
     8101 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
     8161 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
     8221 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
     8281 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
     8341 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
     8401 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
     8461 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
     8521 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
     8581 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
     8641 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
     8701 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
     8761 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
     8821 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
     8881 tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct
     8941 cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct
     9001 cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct
     9061 ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg
     9121 tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc
     9181 gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca
     9241 ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg
     9301 cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc
     9361 ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca
     9421 gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg
     9481 ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat
     9541 agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc
     9601 aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg
     9661 cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca
     9721 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat
     9781 cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc
     9841 acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc
     9901 aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca atctgatcca
     9961 agctcaagct gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10021 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10081 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    10141 ttgcatgcct gcaggtcctg ctgagcctcg acatgttgtc gcaaaattcg ccctggaccc
    10201 gcccaacgat ttgtcgtcac tgtcaaggtt tgacctgcac ttcatttggg gcccacatac
    10261 accaaaaaaa tgctgcataa ttctcggggc agcaagtcgg ttacccggcc gccgtgctgg
    10321 accgggttga atggtgcccg taactttcgg tagagcggac ggccaatact caacttcaag
    10381 gaatctcacc catgcgcgcc ggcggggaac cggagttccc ttcagtgagc gttattagtt
    10441 cgccgctcgg tgtgtcgtag atactagccc ctggggcact tttgaaattt gaataagatt
    10501 tatgtaatca gtcttttagg tttgaccggt tctgccgctt tttttaaaat tggatttgta
    10561 ataataaaac gcaattgttt gttattgtgg cgctctatca tagatgtcgc tataaaccta
    10621 ttcagcacaa tatattgttt tcattttaat attgtacata taagtagtag ggtacaatca
    10681 gtaaattgaa cggagaatat tattcataaa aatacgatag taacgggtga tatattcatt
    10741 agaatgaacc gaaaccggcg gtaaggatct gagctacaca tgctcaggtt ttttacaacg
    10801 tgcacaacag aattgaaagc aaatatcatg cgatcatagg cgtctcgcat atctcattaa
    10861 agcaggactc tagggactag tcccgggtct taattaagtt tgcaagcagc agattacgcg
    10921 cagaaaaaaa ggatctcaag gagatccttt ggatcacaaa aaaaaagccc gctcattagg
    10981 cgggttaaat tcccgggtac tgcagccgat tcattaatgc aggttaacct ggcttatcga
    11041 aattaatacg actcactata gggagaccgg cagatctgat ccctactcca aaaatgtcaa
    11101 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatttcggg
    11161 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtggaaaa
    11221 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatgc
    11281 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg agggaaaaga
    11341 agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag
    11401 ggatgacgca caatcccacc cctactccaa aaatgtcaaa gatactgtct cagaagacca
    11461 aagggctat
//
