LOCUS       sequence-16760-e        1309 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4048
FEATURES             Location/Qualifiers
     source          1..1309
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             247..267
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             289..309
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             331..351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             373..393
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(436..464)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gttactgatc ttctaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
       61 atcactcaca acgatggata cgctaacaac ttggaaatga aataagcttg catgcctgca
      121 ggtcgactag aggatccccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
      181 taacataaca tagaacattt tcaggaggac ccttggaggg taccgagctc agaaaaaatg
      241 actgctccaa agaagaagcg taaggtaccg gcagaaaaaa tgactgctcc aaagaagaag
      301 cgtaaggtac cggcagaaaa aatgactgct ccaaagaaga agcgtaaggt accggcagaa
      361 aaaatgactg ctccaaagaa gaagcgtaag gtaccggtag aaaaaatgag taaaggagaa
      421 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      481 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      541 tttatttgca ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta
      601 actaaccctg attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt
      661 caatgcttct cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
      721 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      781 cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa
      841 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      901 gaagatggaa acattcttgg acacaattgg aatacnacta tanctcacca atgtatacct
      961 catggcagac aaccaaagan tggaatcaag ttgtagttta acntgattta ctaactacta
     1021 tctgatttaa tttcngactc caattaaccc acntgagatg gagcgtcact acnnaccttn
     1081 tcacaatatc cattgggang cctgtcttta cnaancntac tgccncatng cnttcaaatc
     1141 ccacaaaaan acctggcttn ngatngaccc ggggatnctg ggggcactac aacccccctn
     1201 nattnnncnc cnaaaaccgg anccccccnn gattnatnaa cgaangtttc naaatacccg
     1261 gnttcnncna ananccaaan ttnncccccc cnccccccaa ggnttttnt
//
