LOCUS       sequence-167605-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shuttle plasmid for sarA P1-YFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pTH3
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..75)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     670..698
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 attacgaatt cttacttgta cagctcgtcc atgccaagtg taatcccagc agctgttaca
       61 aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattga
      121 taggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt
      181 tgataatggt ctgctagttg aacacctcca tcttcaatgt tgtgtctaat tttgaagtta
      241 gctttgattc cattcttttg tttgtctgct gtgatgtata cattgtgtga gttatagttg
      301 tattccaatt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ttttaactcg
      361 attctattaa caagggtatc accttcaaac ttgacttcag cacgtgtctt gtagttcccg
      421 tcatctttga aaaatatagt tctttcctgt acataacctt cgggcatggc actcttgaaa
      481 aagtcatgct gtttcatatg atctgggtat ctcgcaaagc attgaagacc atatccaaga
      541 gtagtgacaa gtgttggcca tggaacaggt agttttccag tagtgcaaat aagtttaagg
      601 gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca
      661 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct
      721 ttactcataa ataatcatcc tcctaaggta cccggggatc cgatgcatct tgctcgatac
      781 atttgcccga taatatattg atatctaatc tttatttatt atagatatgt tagtcataat
      841 tttgcattaa ataagtttta ttaaatatat ttaatgctct attatttagt taattataac
      901 taattaaaaa tgagaagtaa acaaaaaagt gtttataaaa caaattattc attagacaca
      961 gtgattgtat ttctgggtta gcatttggtt tagtcaaaaa tatcaggcta gcggatcctc
     1021 tagagtcgac ctgcaggcat gcaagctttt aaaaagcaaa
//
