LOCUS       sequence-16761-e        1355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4049
FEATURES             Location/Qualifiers
     source          1..1355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             416..436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             458..478
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             500..520
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             542..562
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(605..633)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngttactga tcttctaact aactattctc atttaaattt tcagagctta aaaatggctg
       61 acntcnctca caacgatgga tacgctaaca acttggaaat gaaataagct tgcatgcctg
      121 caggccttgg tcgactctag acacttttca gctacctaga attacaattg gatatccccg
      181 cctcccaatc cacccaccca gggaaaaaga agggctcgcc gaaaatcaaa gttatctcca
      241 ggctcgcgca tcccaccgag cggttgactt ctctccacca cttttcattt taaccctcgg
      301 ggtacgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
      361 acattttcag gaggaccctt cttggagggt accgagctca gaaaaaatga ctgctccaaa
      421 gaagaagcgt aaggtaccgg cagaaaaaat gactgctcca aagaagaagc gtaaggtacc
      481 ggcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggcagaaa aaatgactgc
      541 tccaaagaag aagcgtaagg taccggtaga aaaaatgagt aaaggagaag aacttttcac
      601 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      661 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      721 tactggaaaa ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga
      781 ttatttaaat tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc
      841 gagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
      901 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacnagacac gtaagtttaa
      961 acagtttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtncaagttt
     1021 gaagggggat ncccttgttn atagaatcng agtttaaang gtnttgattt ttaaagaaaa
     1081 tgggaaacnt ncttggnacc caaattggaa aaacactttn actcccccna ngtttccttc
     1141 tnggcaaaca aanaaaaang ggaatcnagt tgnagtttna ncntgatttt cnaactanct
     1201 antcgnattt aatttccnac tncaaattaa ancnacttgg agaanggngg ntnccntcca
     1261 aacttttcnc aaanttcnca ttggggnggg cngtnntttn aaaaaaantn ttggtnccca
     1321 atnngcnttt naaanaccaa aaaaaancnn tgtct
//
